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PXD000786

PXD000786 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleBMP1/Tolloid-like substrates by iTRAQ degradomics
DescriptionIn this study, we used an iTRAQ-based quantitative proteomic approach to reveal the release of proteolytic fragments from the cell surface or the ECM by BMP-1. Thirty-seven extracellular proteins were found in significantly higher or lower amounts in the conditioned medium of HT1080 cells overexpressing BMP-1 and thus, could be considered as candidate substrates. Strikingly, three of these new candidates (betaglycan, CD109 and neuropilin-1) were TGF-Beta co-receptors, also acting as antagonists when released from the cell surface, and were chosen for further substrate validation.
HostingRepositoryPRIDE
AnnounceDate2014-09-30
AnnouncementXMLSubmission_2014-09-30_00:30:18.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD000786
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterFrederic DELOLME
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListdeamidated residue; monohydroxylated residue; acetylated residue; iTRAQ4plex-116 reporter+balance reagent acylated residue; methylthiolated residue
InstrumentQSTAR
Dataset History
RevisionDatetimeStatusChangeLog Entry
02014-03-03 08:42:50ID requested
12014-09-29 02:28:47announced
22014-09-30 00:30:20announcedUpdated project metadata.
Publication List
Delolme F, Anastasi C, Alcaraz LB, Mendoza V, Vadon-Le Goff S, Talantikite M, Capomaccio R, Mevaere J, Fortin L, Mazzocut D, Damour O, Zanella-Cl, é, on I, Hulmes DJ, Overall CM, Valcourt U, Lopez-Casillas F, Moali C, co-receptor activity by BMP-1/tolloid-like proteases revealed by quantitative iTRAQ proteomics. Cell Mol Life Sci, 72(5):1009-27(2015) [pubmed]
Keyword List
curator keyword: Biomedical
submitter keyword: TFG-Beta, BMP-1, protease, iTRAQ, iTRAQ-ATOMS
Contact List
Frederic Delolme
contact affiliationIBCP-CCMP, CNRS, LYON France
contact emailfrederic.delolme@ibcp.fr
lab head
Frederic DELOLME
contact affiliationCNRS-IBCP
contact emailfrederic.delolme@ibcp.fr
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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