PXD000772

PXD000772 is an original dataset announced via ProteomeXchange.

Title	Temporal and ligand effects on phagosome maturation
Description	Systems biology analysis of temporally-resolved phagosome proteomes following uptake via key phagocytic receptors Macrophages operate at the forefront of innate immunity, and their discrimination of pathogenic versus “self” particles is critical for a number of responses including efficient pathogen killing, antigen presentation and cytokine induction. In order to efficiently phagocytose and destroy the particles, macrophages express an array of receptors to sense and internalise prey particles. In this manuscript, particles conjugated to seven ligands representing pathogen-associated molecular patterns, immune opsonins or apoptotic cell markers were inoculated to murine bone marrow-derived macrophages (BMDMs) and proteomes of isolated phagosomes were accurately quantified among conditions and across a timecourse. While we identified a clear functional differentiation over the three timepoints, only subtle differences were detected between certain ligand-phagosomes, suggesting that triggering of receptors through a single ligand has only mild effects on phagosome proteome and function. This data shows for the first time a systematic time-course analysis of BMDM phagosomes and how phagosome fate is regulated by the receptors triggered for phagocytosis.
HostingRepository	PRIDE
AnnounceDate	2015-09-10
AnnouncementXML	Submission_2015-09-10_06:16:48.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Matthias Trost
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	deamidated residue; monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue; mTRAQ light reporter+balance reagent N6-acylated lysine; mTRAQ light reporter+balance reagent acylated N-terminal
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2014-02-21 01:07:48	ID requested
⏵ 1	2015-09-10 06:16:49	announced

Dill BD, Gierlinski M, H, ä, rtlova A, Arandilla AG, Guo M, Clarke RG, Trost M, Quantitative proteome analysis of temporally resolved phagosomes following uptake via key phagocytic receptors. Mol Cell Proteomics, 14(5):1334-49(2015) [pubmed]

submitter keyword: Phagosome, macrophage, mTRAQ, proteomics, phagocytosis

Matthias Trost
contact affiliation	MRC Protein Phosphorylation and Ubiquitylation Unit, University of Dundee, Dow St, Dundee, DD1 5EH, Scotland, UK
contact email	m.trost@dundee.ac.uk
lab head
Matthias Trost
contact affiliation	MRC Protein Phosphorylation Unit
contact email	m.trost@dundee.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD000772
     1. Label: PRIDE project
     2. Name: Temporal and ligand effects on phagosome maturation