PXD000763

PXD000763 is an original dataset announced via ProteomeXchange.

Title	Identification of inositol 1,4,5-trisphosphate-binding proteins by heparin-agarose affinity purification and LTQ ORBITRAP MS in Oryza sativa
Description	Inositol 1,4,5-trisphosohate (IP3) and its receptors play a pivotal role in calcium signal transduction in mammals. Some fractions of tonoplast and endoplasmic reticulum membranes have high affinity binding activity for IP3, and IP3 can induce Ca2+ release in several plants. However, no homologues of mammalian IP3 receptors have been found in plants. In this study, we isolated the microsomal fractions from rice cells in suspension culture and further obtained putative IP3-binding proteins by heparin-agarose affinity purification. The IP3 binding activities of these protein fractions were determined by [3H]IP3 binding assay. SDS-PAGE and MS analysis were then performed to characterise these proteins. We have identified 297 proteins from the eluates of heparin-agarose column chromatography, which will provide insight into the IP3 signalling pathways in plants.
HostingRepository	PRIDE
AnnounceDate	2014-09-25
AnnouncementXML	Submission_2014-09-25_05:40:30.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Shengcheng Han
SpeciesList	scientific name: Rice(O. sativa); NCBI TaxID: 4530;
ModificationList	monohydroxylated residue; acetylated residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2014-02-17 07:45:04	ID requested
⏵ 1	2014-09-25 05:40:31	announced

Nie Y, Huang F, Dong S, Li L, Gao P, Zhao H, Wang Y, Han S, Identification of inositol 1,4,5-trisphosphate-binding proteins by heparin-agarose affinity purification and LTQ ORBITRAP MS in Oryza sativa. Proteomics, 14(20):2335-8(2014) [pubmed]

curator keyword: Biological

submitter keyword: Heparin-agarose, IP3, IP3 binding protein, LTQ ORBITRAP MS, Oryza sativa

Shengcheng Han
contact affiliation	Beijing Key Laboratory of Gene Resource and Molecular Development, College of Life Sciences, Beijing Normal University, Beijing China
contact email	schan@bnu.edu.cn
lab head
Shengcheng Han
contact affiliation	Beijing Normal University
contact email	schan@bnu.edu.cn
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD000763
     1. Label: PRIDE project
     2. Name: Identification of inositol 1,4,5-trisphosphate-binding proteins by heparin-agarose affinity purification and LTQ ORBITRAP MS in Oryza sativa