PXD000761

PXD000761 is an original dataset announced via ProteomeXchange.

Title	Phosphoproteome profiling of toll-like receptor response to different ligand stimulation in macrophages identifies differences in global phosphorylation dynamics
Description	Toll-like receptors are among the first sensors that detect and drive immune responses to pathogens. Macrophages that encounter a pathogen are usually not stimulated through one TLR but by a combination of these receptors engaged by distinct ligands produced by the microbe. As a first step to understanding the integrated signaling under such complex conditions, we have investigated the differences in the phosphoprotein signaling cascades triggered by individual TLR4, TLR2 and TLR7 ligands using a single responding cell population. We performed a global quantitative and early post-stimulation kinetic analysis of the mouse macrophage phosphoproteome using stable isotope labeling with amino acids coupled to phosphopeptide enrichment and high-resolution mass spectrometry.
HostingRepository	PRIDE
AnnounceDate	2014-12-02
AnnouncementXML	Submission_2014-12-02_00:31:09.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Aleksandra Nita-Lazar
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	iodoacetamide derivatized residue; phosphorylated residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2014-02-17 02:49:15	ID requested
1	2014-06-09 02:33:03	announced
2	2014-07-25 01:52:05	announced	Updated project metadata.
3	2014-08-08 06:04:39	announced	Updated project metadata.
⏵ 4	2014-12-02 00:31:10	announced	Updated project metadata.

Sjoelund V, Smelkinson M, Nita-Lazar A, Phosphoproteome profiling of the macrophage response to different toll-like receptor ligands identifies differences in global phosphorylation dynamics. J Proteome Res, 13(11):5185-97(2014) [pubmed]

curator keyword: Biomedical, Biological

submitter keyword: Mouse, macrophage, TLR4, TLR2, TLR7, phosphoproteomics

Aleksandra Nita-Lazar
contact affiliation	NIH, NIAID, LSB
contact email	nitalarau@niaid.nih.gov
lab head
Aleksandra Nita-Lazar
contact affiliation	NIAID
contact email	nitalazarau@niaid.nih.gov
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD000761
     1. Label: PRIDE project
     2. Name: Phosphoproteome profiling of toll-like receptor response to different ligand stimulation in macrophages identifies differences in global phosphorylation dynamics