PXD000747

PXD000747 is an original dataset announced via ProteomeXchange.

Title	Copy number analysis of the murine platelet proteome spanning the complete abundance range
Description	Knowledge of the identity and quantity of expressed proteins of a cell type is a prerequisite for a complete understanding of its molecular functions. Mass spectrometry (MS)-based proteomics has allowed the identification of the entire protein complement of yeast and the close-to-complete set of proteins expressed in mammalian cell lines. Using recent technological advances we here characterize the proteome of murine platelets, key actors in mediating hemostasis and thrombosis. We accurately measured the absolute protein concentrations of thirteen platelet proteins by SILAC-Protein Epitope Signature Tags (PrESTs) and used them as reference points to estimate the copy number of all proteins of the platelet proteome. To distinguish contaminants such as plasma or erythrocyte proteins from true platelet proteins, we monitored protein abundance profiles across multiple purification steps. In total, we absolutely quantified 4,400 platelet proteins, with estimated copy numbers ranging from less than ten to about a million per cell. Stoichiometries derived from our data correspond well with previous studies. Our study provides a close-to-complete reference map of platelet proteins, which will be useful to the community, for instance for interpreting mouse models of human platelets diseases.
HostingRepository	PRIDE
AnnounceDate	2015-08-18
AnnouncementXML	Submission_2015-08-18_07:32:59.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Nadin Neuhauser
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2014-02-07 06:12:46	ID requested
⏵ 1	2015-08-18 07:33:00	announced

Zeiler M, Moser M, Mann M, Copy number analysis of the murine platelet proteome spanning the complete abundance range. Mol Cell Proteomics, 13(12):3435-45(2014) [pubmed]

curator keyword: Biomedical, Biological

submitter keyword: murine, platelet

Matthias Mann
contact affiliation	Max Planck Institute of Biochemistry
contact email	mmann@biochem.mpg.de
lab head
Nadin Neuhauser
contact affiliation	Max Planck Institute of Biochemistry
contact email	neuhause@biochem.mpg.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD000747
     1. Label: PRIDE project
     2. Name: Copy number analysis of the murine platelet proteome spanning the complete abundance range