PXD000746

PXD000746 is an original dataset announced via ProteomeXchange.

Title	Proteomics of Fuchs’ Endothelial Corneal Dystrophy indicate altered assembly and modulation of the extracellular matrix in Descemet’s membrane
Description	Fuchs’ endothelial corneal dystrophy is major corneal disorder in the western world affecting the innermost part of the cornea, which leads to visual impairment. The morphological changes observed in Fuchs’ endothelial corneal dystrophy is well described, however, much less in known of the pathology at the molecular level. As the morphological changes observed in the cornea is profound in the extracellular matrix we sought to determine in protein profiles and changes herein in the Descement’s membrane and endothelium layer of Fuchs’ endothelial conrneal dystrophy patients when compared to healthy control tissue. Using the extracted ion chromatogram label-free MS based quantification method we quantified approximately the 50 most abundant proteins of the Descemet’s membrane and endothelial layer in in patient and control tissue. In addition, using the isobaric tag for relative and absolute quantification MS method resulted in a total of 22 regulated proteins of which the majority were extracellular proteins known to be involved in proper assembly and modulation of the basement membrane in other tissues. Many of the regulated proteins were furthermore among the most abundant proteins quantified. The two MS methods performed here suggest altered arrangement of the extracellular matrix in Fuchs’ endothelial corneal dystrophy and provide new candidate proteins that may be involved in molecular mechanism of this disease.
HostingRepository	PRIDE
AnnounceDate	2014-05-27
AnnouncementXML	Submission_2014-05-27_03:04:14.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD000746
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Ebbe Toftgaard Poulsen
SpeciesList	scientific name: Homo sapiens (human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iTRAQ4plex-116 reporter+balance reagent acylated residue; iodoacetamide derivatized residue; 2: 4
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2014-02-07 03:43:02	ID requested
⏵ 1	2014-05-27 03:04:15	announced

Poulsen ET, Dyrlund TF, Runager K, Scavenius C, Krogager TP, H, ø, jrup P, Th, ø, gersen IB, Sanggaard KW, Vorum H, Hjortdal J, Enghild JJ, Proteomics of Fuchs' endothelial corneal dystrophy support that the extracellular matrix of Descemet's membrane is disordered. J Proteome Res, 13(11):4659-67(2014) [pubmed]

submitter keyword: Fuchs' endothelial corneal dystrophy, XIC quantification, iTRAQ quantification

Jan Johannes Enghild
contact affiliation	Department of Molecular Biology and Genetics, Aarhus University, Denmark
contact email	jje@mb.au.dk
lab head
Ebbe Toftgaard Poulsen
contact affiliation	Department of Molecular Biology and Genetics
contact email	etp@mb.au.dk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD000746
     1. Label: PRIDE project
     2. Name: Proteomics of Fuchs’ Endothelial Corneal Dystrophy indicate altered assembly and modulation of the extracellular matrix in Descemet’s membrane