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PXD000743

PXD000743 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleInsight into the protein composition of immunoglobulin light chain deposits of eyelid, orbital, and conjunctival amyloidosis.
DescriptionAmyloidosis is a disorder characterized by the formation of extracellular amyloid deposits. Immunoglobulin light-chain amyloidosis the most common form of amyloidosis can appear as a local disorder presented with mild symptoms or as a life threatening systemic disease. Identification of the proteins forming amyloid fibrils is essential for the diagnosis of the disease and knowledge about the overall protein composition of the deposits may lead to a larger understanding of the deposition events thereby facilitating a more detailed picture of the molecular pathology. In this study, we investigated the protein composition of AL amyloid deposits isolated from human eyelid, conjunctival and orbital specimens. Deposits and internal control tissue (patient tissue without apparent deposits) were procured by laser capture microdissection. Proteins in the captured amyloid and control samples were identified by liquid chromatography tandem mass spectrometry and subsequently quantified using the label-free mass spectrometry quantification method exponential modified Protein Abundance Index. Immunoglobulin light chain kappa or lambda was revealed to be the most predominant protein in the amyloid deposits. In addition, the protein profiles identified apolipoprotein E and serum amyloid P component to be associated with the immunoglobulin light chain deposits across all three tissues analyzed. The method used in this study provides high sensitivity and specificity of typing amyloidosis and may provide additional information on the pathology of amyloidosis.
HostingRepositoryPRIDE
AnnounceDate2019-02-25
AnnouncementXMLSubmission_2019-02-25_04:17:18.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD000743
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterNadia Sukusu Nielsen
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentTripleTOF 5600
Dataset History
RevisionDatetimeStatusChangeLog Entry
02014-02-06 05:33:38ID requested
12014-05-29 01:59:14announced
22014-07-24 05:16:33announcedUpdated project metadata.
32019-02-25 04:17:19announcedUpdated publication reference for PubMed record(s): 26500418.
Publication List
Nielsen NS, Poulsen ET, Klintworth GK, Enghild JJ, Insight into the Protein Composition of Immunoglobulin Light Chain Deposits of Eyelid, Orbital and Conjunctival Amyloidosis. J Proteomics Bioinform, Suppl 8():(2014) [pubmed]
Keyword List
curator keyword: Biomedical
submitter keyword: Amyloidosis, conjunctiva, eyelid, orbit, laser capture microdissection, mass spectrometry
Contact List
Jan J. Enghild
contact affiliationDepartment of Molecular Biology and Genetics, Aarhus University, Denmark
contact emailjje@mb.au.dk
lab head
Nadia Sukusu Nielsen
contact affiliationAarhus University
contact emailnsn@mb.au.dk
dataset submitter
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Dataset FTP location
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