PXD000738

PXD000738 is an original dataset announced via ProteomeXchange.

Title	SILAC-based proteomic quantification of chemoattractant induced cytoskeleton dynamics on a second to minute timescale
Description	Cytoskeletal dynamics during cell behaviours ranging from endocytosis and exocytosis to cell division and movement is controlled by a complex network of signalling pathways, the full details of which are as yet unresolved. Here we show that SILAC-based proteomic methods can be used to characterise the rapid chemoattractant induced dynamic changes in the actin-myosin cytoskeleton and regulatory elements on a proteome wide scale with a second to minute timescale resolution. This approach provides novel insights in the ensemble kinetics of key cytoskeletal constituents and association of known and novel identified binding proteins. We validate the proteomic data by detailed microscopy based analysis of in vivo translocation dynamics for key signalling factors. This rapid large-scale proteomic approach may be applied to other situations where highly dynamic changes in complex cellular compartments are expected to play a key role.
HostingRepository	PRIDE
AnnounceDate	2014-07-24
AnnouncementXML	Submission_2014-07-24_05:17:00.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Vackar Afzal
SpeciesList	scientific name: Dictyostelium discoideum (Slime mold); NCBI TaxID: 44689;
ModificationList	oxidized residue; phosphorylated residue; S-carboxamidomethyl-L-cysteine; Acetyl; N-acylated residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2014-02-04 07:35:04	ID requested
1	2014-02-26 04:12:09	announced
2	2014-03-05 01:20:04	announced	Updated publication reference for PubMed record(s): 24569529.
⏵ 3	2014-07-24 05:17:01	announced	Updated project metadata.

Sobczyk GJ, Wang J, Weijer CJ, SILAC-based proteomic quantification of chemoattractant-induced cytoskeleton dynamics on a second to minute timescale. Nat Commun, 5():3319(2014) [pubmed]

curator keyword: Biological

submitter keyword: Silac, Cytoskeleton

Cornelis J Weijer
contact affiliation	College of Life Sciences, University of Dundee
contact email	C.j.weijer@dundee.ac.uk
lab head
Vackar Afzal
contact affiliation	School of Life Sciences
contact email	v.z.afzal@dundee.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2014/02/PXD000738

PRIDE project URI

• PRIDE
  1. PXD000738
     1. Label: PRIDE project
     2. Name: SILAC-based proteomic quantification of chemoattractant induced cytoskeleton dynamics on a second to minute timescale