PXD000736

PXD000736 is an original dataset announced via ProteomeXchange.

Title	Altered brain pathways following Chikungunya virus infection
Description	A kinetic analysis of host proteome modifications in the brain of CHIKV-infected mice sampled before and after the onset of clinical symptoms was performed. The combination of 2D-DIGE and iTRAQ proteomic approaches, followed by mass spectrometry protein identification, revealed 177 significantly differentially expressed proteins. This kinetic analysis was characterized by a dramatic down-regulation of proteins prior the appearance of the clinical symptoms followed by an increase expression of a large part of these proteins in the acute phase of symptoms. Bioinformatic analysis of the protein dataset allowed to identify major biological processes altered during the time course of CHIKV infection such as integrin signaling and cytoskeleton dynamics, endosome machinery and receptor recycling related to virus transport and synapse function, regulation of gene expression, and ubiquitin-proteasome pathway. This work gives new information on putative mechanisms that could be associated with severe neurological CHIKV infection-mediated disease. It also describes possible markers or targets that can be used to develop diagnostic and/or antiviral tools.
HostingRepository	PRIDE
AnnounceDate	2015-07-11
AnnouncementXML	Submission_2015-07-11_00:31:18.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Lionel Almeras
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	iTRAQ8plex-116 reporter+balance reagent acylated residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2014-02-03 02:46:12	ID requested
1	2015-07-08 02:57:57	announced
⏵ 2	2015-07-11 00:31:19	announced	Updated project metadata.

Fraisier C, Koraka P, Belghazi M, Bakli M, Granjeaud S, Pophillat M, Lim SM, Osterhaus A, Martina B, Camoin L, Almeras L, Kinetic analysis of mouse brain proteome alterations following Chikungunya virus infection before and after appearance of clinical symptoms. PLoS One, 9(3):e91397(2014) [pubmed]

curator keyword: Biomedical, Biological

submitter keyword: alphavirus, Chikungunya virus, pathways modified, pathogenesis, mouse, brain, infection, iTRAQ, 2D-DIGE

Pr. Christophe ROGIER
contact affiliation	Unit?? de recherche en biologie et ??pid??miologie parasitaires (URBEP) ??? UMR6236 ??? IFR48, Institut de Recherche Biom??dicale des Arm??es (IRBA), antenne Marseille, URMITE UMR 6236, GSBdD de Marseille Aubagne, 111 avenue de la corse BP 40026, 13568 Marseille cedex 02, France.
contact email	christophe.rogier@gmail.com
lab head
Lionel Almeras
contact affiliation	Life Sciences
contact email	almeras.lionel@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD000736
     1. Label: PRIDE project
     2. Name: Altered brain pathways following Chikungunya virus infection