Updated project metadata. Leishmania donovani is a kinetoplastid protozoan which causes Kala-azar or visceral leishmaniasis.Leishmania possess glycosomes that are unique and specialized subcellular microbody organelles. Glycosomes are known to harbor most peroxisomal enzymes and in addition they also possess glycolytic enzymes. In the present study, we carried out proteomic profiling of purified glycosomes isolated from L. donovani promastigotes using high resolution mass spectrometry. The majority of identified proteins are involved in metabolic processes such as carbohydrate, lipid and nucleic acid metabolism. Our present proteomic analysis is the most comprehensive study till date to map the proteome of L. donovaniglycosomes.