PXD000711

PXD000711 is an original dataset announced via ProteomeXchange.

Title	Comparative proteomics of two life cycle stages of stable isotope-labeled Trypanosoma brucei reveals novel components of the parasite's host adaptation machinery.
Description	Trypanosoma brucei developed a sophisticated life cycle to adapt to different host environments. Although developmental differentiation of T. brucei has been the topic of intensive research for decades, the mechanisms responsible for adaptation to different host environments are not well understood. We developed stable isotope labeling by amino acids in cell culture in trypanosomes to compare the proteomes of two different life cycle stages. Quantitative comparison of 4364 protein groups identified many proteins previously not known to be stage-specifically expressed. The identification of stage-specific proteins helps to understand how parasites adapt to different hosts and provides new insights into differences in metabolism, gene regulation, and cell architecture. A DEAD-box RNA helicase, which is highly up-regulated in the bloodstream form of this parasite and which is essential for viability and proper cell cycle progression in this stage is described as an example.
HostingRepository	PRIDE
AnnounceDate	2015-02-17
AnnouncementXML	Submission_2015-02-17_02:57:47.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	F Butter
SpeciesList	scientific name: Trypanosoma brucei; NCBI TaxID: 5691;
ModificationList	6x(13)C: 4x(15)N labeled L-arginine; monohydroxylated residue; iodoacetamide derivatized residue; 6x(13)C: 2x(15)N labeled L-lysine; N-acetylated residue
Instrument	LTQ Orbitrap; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2014-01-24 00:39:42	ID requested
1	2014-01-28 08:34:23	announced
2	2014-04-16 03:38:48	announced	Updated publication reference for PubMed record(s): 23090971, 24552149.
3	2014-07-24 05:16:38	announced	Updated project metadata.
⏵ 4	2015-02-17 02:57:48	announced	Updated project metadata.

Ericson M, Janes MA, Butter F, Mann M, Ullu E, Tschudi C, On the extent and role of the small proteome in the parasitic eukaryote Trypanosoma brucei. BMC Biol, 12():14(2014) [pubmed]

Butter F, Bucerius F, Michel M, Cicova Z, Mann M, Janzen CJ, Comparative proteomics of two life cycle stages of stable isotope-labeled Trypanosoma brucei reveals novel components of the parasite's host adaptation machinery. Mol Cell Proteomics, 12(1):172-9(2013) [pubmed]

ProteomeXchange project tag: PRIME-XS Project

curator keyword: Biological

submitter keyword: trypanosomes

Matthias Mann
contact affiliation	MPI for Biochemistry
contact email	mann@biochem.mpg.de
lab head
F Butter
contact affiliation	Dept. of Proteomics and Signal Transduction
contact email	f.butter@imb-mainz.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2014/01/PXD000711

PRIDE project URI

• PRIDE
  1. PXD000711
     1. Label: PRIDE project
     2. Name: Comparative proteomics of two life cycle stages of stable isotope-labeled Trypanosoma brucei reveals novel components of the parasite's host adaptation machinery.