PXD000695

PXD000695 is an original dataset announced via ProteomeXchange.

Title	In-depth proteomic analyses of ovarian cancer cell line exosomes reveals differential enrichment of functional categories compared to the NCI 60 proteome.
Description	Molecular communication between cancer cells and its stromal microenvironment is a key factor for cancer progression. Alongside classic secretory pathways, it has recently been proposed that small membranous vesicles are alternative mediators of intercellular communication. Exosomes carry an effector-rich proteome with the ability to modulate various functional properties of the recipient cell. In this study, exosomes isolated from four epithelial ovarian cancer cell lines (OVCAR3, OVCAR433, OVCAR5 and SKOV3) were characterized using mass spectrometry-based proteomics. Using an optimized workflow consisting of efficient exosome solubilization and the latest generation of proteomic instrumentation, we demonstrate improved detection depth. Systematic comparison of our cancer cell line exosome proteome against public data (ExoCarta) and the recently published NCI 60 proteome revealed enrichment of functional categories related to signaling biology and biomarker discovery.
HostingRepository	PRIDE
AnnounceDate	2014-07-24
AnnouncementXML	Submission_2014-07-24_03:49:26.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Thomas Kislinger
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2014-01-21 01:29:51	ID requested
1	2014-01-23 07:12:00	announced
⏵ 2	2014-07-24 03:49:26	announced	Updated project metadata.

Sinha A, Ignatchenko V, Ignatchenko A, Mejia-Guerrero S, Kislinger T, In-depth proteomic analyses of ovarian cancer cell line exosomes reveals differential enrichment of functional categories compared to the NCI 60 proteome. Biochem Biophys Res Commun, 445(4):694-701(2014) [pubmed]

curator keyword: Biomedical

submitter keyword: Exosome, Ovarian Cancer, Proteomics

Thomas Kislinger
contact affiliation	Princess Margaret Cancer Center, Toronto, ON, Canada.
contact email	thomas.kislinger@utoronto.ca
lab head
Thomas Kislinger
contact affiliation	Medical Biophysics
contact email	thomas.kislinger@utoronto.ca
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD000695
     1. Label: PRIDE project
     2. Name: In-depth proteomic analyses of ovarian cancer cell line exosomes reveals differential enrichment of functional categories compared to the NCI 60 proteome.