PXD000694

PXD000694 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of Giardia lamblia ESV and PV organelle enriched fractions
Description	The protozoan Giardia lamblia is a unique biological model to investigate minimal cellular mechanisms because it is an early‐diverged eukaryote that has undergone reductive evolution and has minimized or lost organelles such as mitochondria, peroxisomes, the endo-lysosomal system and a Golgi apparatus. Giardia trophozoites reside in the host intestines where they largely depend on scavenging metabolites from the surrounding through endocytosis and/or pinocytosis. A canonical endo‐lysosomal system however is inexistent, but small peripheral vesicles (PVs) have been described which have partial endocytic characteristics but lack endocytic hallmarks: (i) unlike the dynamic multivesicular endo-lysosomal system in mammalian cells, PVs have a fixed location at the cell periphery and show no lateral cargo exchange; (ii) PVs lack maturation-specific markers. For transmission to a new host, motile trophozoites differentiate to cysts and thus secrete a protective cyst wall (CW). Giardia however has no Golgi or to organize regulated secretion of CW material. Instead, differentiating cells generate specialized organelles termed encystation‐specific vesicles (ESVs), being stage-regulated Golgi relic organelles dedicated to post‐translational maturation of the CW material. Basic sorting processes in maturing ESVs have been identified recently, but underlying mechanisms remain unknown. To define the first proteome of ESVs and PVs, we used a new strategy combining flow cytometry-based organelle sorting with in silico filtration of mass spectrometry data.
HostingRepository	PRIDE
AnnounceDate	2014-07-24
AnnouncementXML	Submission_2014-07-24_03:49:16.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD000694
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Adrian Hehl
SpeciesList	scientific name: Giardia intestinalis (strain ATCC 50803 / WB clone C6) (Giardia lamblia); NCBI TaxID: 184922;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2014-01-21 01:15:48	ID requested
1	2014-03-11 07:12:34	announced
2	2014-04-22 03:51:17	announced	Updated publication reference for PubMed record(s): 24732305.
⏵ 3	2014-07-24 03:49:17	announced	Updated project metadata.

Wampfler PB, Tosevski V, Nanni P, Spycher C, Hehl AB, Proteomics of secretory and endocytic organelles in Giardia lamblia. PLoS One, 9(4):e94089(2014) [pubmed]

curator keyword: Biomedical, Biological

submitter keyword: Giardia, encystation, endocytosis, flow cytometry assisted organelle sorting, organelle proteomics

Adrian B. Hehl
contact affiliation	Institute of Parasitology, University of Zurich, Switzerland
contact email	adrian.hehl@access.uzh.ch
lab head
Adrian Hehl
contact affiliation	Institute of Parasitology
contact email	adrian.hehl@access.uzh.ch
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2014/03/PXD000694

PRIDE project URI

• PRIDE
  1. PXD000694
     1. Label: PRIDE project
     2. Name: Proteomic analysis of Giardia lamblia ESV and PV organelle enriched fractions