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PXD000684

PXD000684 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteomics of Pseudomonas aeruginosa PAO1 and PA14
DescriptionRecent studies have shown that the concentrations of proteins expressed from orthologous genes are often conserved across organisms, and to a greater extent than the abundances of the corresponding mRNAs. However, such studies have not distinguished between evolutionary (e.g., sequence divergence) and environmental (e.g., growth condition) effects on the regulation of steady-state protein and mRNA abundances. Here we systematically investigated the transcriptome and proteome of two closely related Pseudomonas aeruginosa strains, PAO1 and PA14, under identical experimental conditions, thus controlling for environmental effects. For 703 genes observed by both shotgun proteomics and microarray experiments, we find that the protein-to-mRNA ratios are highly correlated between orthologous genes in the two strains, to an extent comparable to protein and mRNA abundances. In spite of this high molecular similarity between PAO1 and PA14, we found that several metabolic, virulence, and antibiotic resistance genes are differentially expressed between the two strains, mostly at the protein but not at the mRNA level. Our data demonstrate that post-transcriptional regulation is important for understanding the discordance between mRNA and protein abundance.
HostingRepositoryPRIDE
AnnounceDate2014-05-08
AnnouncementXMLSubmission_2014-05-08_03:59:54.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterTaejoon Kwon
SpeciesList scientific name: Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228); NCBI TaxID: 208964; scientific name: Pseudomonas aeruginosa PA14; NCBI TaxID: 652611;
ModificationListmonohydroxylated residue
InstrumentLTQ Orbitrap
Dataset History
RevisionDatetimeStatusChangeLog Entry
02014-01-15 02:18:30ID requested
12014-05-08 03:59:55announced
Publication List
Kwon T, Huse HK, Vogel C, Whiteley M, Marcotte EM, Protein-to-mRNA ratios are conserved between Pseudomonas aeruginosa strains. J Proteome Res, 13(5):2370-80(2014) [pubmed]
Keyword List
submitter keyword: Pseudomonas aeruginosa, Synthetic Cystic Fibrosis Medium, MSblender
Contact List
Edward Marcotte
contact affiliationUniversity of Texas at Austin
contact emailedward.marcotte@gmail.com
lab head
Taejoon Kwon
contact affiliationUniversity of Texas at Austin
contact emailtaejoon.kwon@marcottelab.org
dataset submitter
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Dataset FTP location
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