PXD000684

PXD000684 is an original dataset announced via ProteomeXchange.

Title	Proteomics of Pseudomonas aeruginosa PAO1 and PA14
Description	Recent studies have shown that the concentrations of proteins expressed from orthologous genes are often conserved across organisms, and to a greater extent than the abundances of the corresponding mRNAs. However, such studies have not distinguished between evolutionary (e.g., sequence divergence) and environmental (e.g., growth condition) effects on the regulation of steady-state protein and mRNA abundances. Here we systematically investigated the transcriptome and proteome of two closely related Pseudomonas aeruginosa strains, PAO1 and PA14, under identical experimental conditions, thus controlling for environmental effects. For 703 genes observed by both shotgun proteomics and microarray experiments, we find that the protein-to-mRNA ratios are highly correlated between orthologous genes in the two strains, to an extent comparable to protein and mRNA abundances. In spite of this high molecular similarity between PAO1 and PA14, we found that several metabolic, virulence, and antibiotic resistance genes are differentially expressed between the two strains, mostly at the protein but not at the mRNA level. Our data demonstrate that post-transcriptional regulation is important for understanding the discordance between mRNA and protein abundance.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:18:34.094.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Taejoon Kwon
SpeciesList	scientific name: Pseudomonas aeruginosa PA14; NCBI TaxID: 652611; scientific name: Pseudomonas aeruginosa PAO1; NCBI TaxID: 208964;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2014-01-15 02:18:30	ID requested
1	2014-05-08 03:59:55	announced
⏵ 2	2024-10-22 04:18:35	announced	2024-10-22: Updated project metadata.

Kwon T, Huse HK, Vogel C, Whiteley M, Marcotte EM, Protein-to-mRNA ratios are conserved between Pseudomonas aeruginosa strains. J Proteome Res, 13(5):2370-80(2014) [pubmed]

curator keyword: Biological

submitter keyword: Synthetic Cystic Fibrosis Medium,Pseudomonas aeruginosa, MSblender

Edward Marcotte
contact affiliation	University of Texas at Austin
contact email	edward.marcotte@gmail.com
lab head
Taejoon Kwon
contact affiliation	UNIST
contact email	tkwon@unist.ac.kr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2014/05/PXD000684

PRIDE project URI

• PRIDE
  1. PXD000684
     1. Label: PRIDE project
     2. Name: Proteomics of Pseudomonas aeruginosa PAO1 and PA14