PXD000677

PXD000677 is an original dataset announced via ProteomeXchange.

Title	14-3-3 affinity capture to identify new targets of 14-3-3 signaling during dsRNA-stimulation in human keratinocytes.
Description	We have combined two proteomic approaches, phosphoproteomics and 14-3-3 affinity capture, to examine the signaling pathways in which 14-3-3 proteins participate and to identify new targets of 14-3-3 signaling during dsRNA-stimulation.
HostingRepository	PRIDE
AnnounceDate	2014-10-23
AnnouncementXML	Submission_2014-10-23_02:53:00.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tuula Nyman
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iTRAQ4plex-116 reporter+balance reagent acylated residue; methylthiolated residue
Instrument	QSTAR

Revision	Datetime	Status	ChangeLog Entry
0	2014-01-09 06:17:06	ID requested
1	2014-08-05 05:28:28	announced
2	2014-08-06 00:30:31	announced	Updated project metadata.
⏵ 3	2014-10-23 02:53:01	announced	Updated project metadata.

Ö, hman T, S, ö, derholm S, Hintsanen P, V, ä, lim, ä, ki E, Lietz, é, n N, MacKintosh C, Aittokallio T, Matikainen S, Nyman TA, Phosphoproteomics combined with quantitative 14-3-3-affinity capture identifies SIRT1 and RAI as novel regulators of cytosolic double-stranded RNA recognition pathway. Mol Cell Proteomics, 13(10):2604-17(2014) [pubmed]

curator keyword: Biomedical

submitter keyword: Human, HaCaT keratinocytes, iTRAQ, LC-MS/MS

Tuula A Nyman
contact affiliation	Institute of Biotechnology, University of Helsinki, Finland
contact email	tuula.nyman@helsinki.fi
lab head
Tuula Nyman
contact affiliation	Institute of Biotechnology
contact email	tuula.nyman@helsinki.fi
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2014/08/PXD000677

PRIDE project URI

• PRIDE
  1. PXD000677
     1. Label: PRIDE project
     2. Name: 14-3-3 affinity capture to identify new targets of 14-3-3 signaling during dsRNA-stimulation in human keratinocytes.