PXD000660

PXD000660 is an original dataset announced via ProteomeXchange.

Title	TAILS with Arabidopsis thaliana wilde typ, tic56-1and ppi2
Description	Aim was to analyse the in vivo start position of proteins in the import-mutant plant lines tic56-1 and ppi2 in contrast to wild type. Therefor we enriched N-terminal peptides originating from total protein of the three plant lines and detect them by mass spectrometry. This method is called TAILS and was described by Doucet et al. 2011 previously. For this analysis two experiments were done, further called TAILS1 and TAILS2. In TAILS1 total soluble protein from wild type and tic56-1 were analysed. N-termini of the wild type were labelled using normal formaldehyde during N-termini from tic56-1 were labelled with heavy formaldehyde. After labelling the two samples were combined and afterward treaded and measured together. In TAILS2 also membrane proteins were solubilized and additionally total protein from ppi2 plants was analysed. In this experiment the protein samples were treaded in the whole procedure separately and all three samples were labelled using normal formaldehyde. For peptide identification the raw-data were analysed using the Proteome Discoverer (search engine: SEQUEST) as well as MaxQuant (search engine: Andromeda). As variable modifications N-acetylation and methionine oxidation and as fixed modification carbamidomethylation of cysteins and dimethylation of lysine residues and N-termini (TAILS1: normal for wild type and heavy for tic56-1 sample) were allowed. For the Proteome Discoverer analysis the peptides diverse in the isotope labels (TAILS1) or the blocked N termini (N-terminal acetylation or dimethylation) were identified in distinct searches.
HostingRepository	PRIDE
AnnounceDate	2015-03-20
AnnouncementXML	Submission_2015-03-20_01:30:24.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sacha Baginsky
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue; 2x(13)C: 4x(2)H labeled dimethylated residue; dimethylated residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2013-12-19 02:13:21	ID requested
1	2015-02-03 03:40:02	announced
⏵ 2	2015-03-20 01:30:26	announced	Updated project metadata.

K, ö, hler D, Montandon C, Hause G, Majovsky P, Kessler F, Baginsky S, Agne B, Characterization of chloroplast protein import without Tic56, a component of the 1-megadalton translocon at the inner envelope membrane of chloroplasts. Plant Physiol, 167(3):972-90(2015) [pubmed]

curator keyword: Biological

submitter keyword: TAILS, plastidic import, ppi2, tic56-1

Sacha Baginsky
contact affiliation	Institut fuer Biochemie und Biotechnologie
contact email	pflanzen@biochemtech.uni-halle.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD000660
     1. Label: PRIDE project
     2. Name: TAILS with Arabidopsis thaliana wilde typ, tic56-1and ppi2