PXD000653

PXD000653 is an original dataset announced via ProteomeXchange.

Title	CSF proteome mapping: Human cerebrospinal fluid LC-MS/MS, Part 3
Description	3 mL CSF from a pool of 21 neurologically healthy donors was in-solution trypsin digested followed by Solid-phase enrichment of N-linked glycopeptides (SPEG) using hydrazide modified beads. Glycopeptides were further fractionated into 20 fractions by mixed-mode reversed phase anion exchange (MM(RP-AX) using a Promix MP column. Each fraction was analyzed separately by LC-MS/MS on an Orbitrap Velos Pro mass spectrometer. Resulting data was searched using SeachGui, summarized in PeptideShaker and exported to the CSF-PR.
HostingRepository	PRIDE
AnnounceDate	2014-10-23
AnnouncementXML	Submission_2014-10-23_02:47:40.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD000653
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Astrid Guldbrandsen
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	2-pyrrolidone-5-carboxylic acid (Glu): -18.010565; acetylated residue: 42.010565; iodoacetamide derivatized amino-terminal residue: 57.021464; 2-pyrrolidone-5-carboxylic acid (Gln): -17.026549; monohydroxylated residue: 15.994915; deamidated residue: 0.984016
Instrument	LTQ Orbitrap; instrument model: LTQ-Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2013-12-18 04:16:43	ID requested
1	2014-08-04 08:00:30	announced
⏵ 2	2014-10-23 02:47:41	announced	Updated project metadata.

Guldbrandsen A, Vethe H, Farag Y, Oveland E, Garberg H, Berle M, Myhr KM, Opsahl JA, Barsnes H, Berven FS, In-depth characterization of the cerebrospinal fluid (CSF) proteome displayed through the CSF proteome resource (CSF-PR). Mol Cell Proteomics, 13(11):3152-63(2014) [pubmed]

curator keyword: Biomedical

submitter keyword: Cerebrospinal fluid, Homo Sapiens, LC-MS/MS

Astrid Guldbrandsen
contact affiliation	PROBE
contact email	astrid.guldbrandsen@biomed.uib.no
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2014/08/PXD000653

PRIDE project URI

• PRIDE
  1. PXD000653
     1. Label: PRIDE project
     2. Name: CSF proteome mapping: Human cerebrospinal fluid LC-MS/MS, Part 3