The workflow for targeted proteome survey of proteins encoded by chromosome 18 was performed in three steps. SRM scouting enabled to acquire the preliminary data about the presence of proteotypic peptides in the depleted plasma (HPL) and HepG2 cells (HG2). For the observed signals from target peptides the developed acquisition and qualitative analysis methods were checked and confirmed by comparison of purified non-labeled synthesized analogues for the endogenous peptides with the data set named Scout. The second dataset is acquired from synthesized peptides and named QC.