PXD000646

PXD000646 is an original dataset announced via ProteomeXchange.

Title	Wheat leaf phosphoproteome under drought stress
Description	Waxy starch has an important influence on the qualities of breads. Generally, grain weight and yield in waxy wheat (Triticum aestivum L.) are significantly lower than in bread wheat. In this study, we performed the first proteomic and phosphoproteomic analyses of starch granule-binding proteins by comparing the waxy wheat cultivar Shannong 119 and the bread wheat cultivar Nongda 5181. These results indicate that reduced amylose content does not affect amylopectin synthesis, but it causes significant reduction of total starch biosynthesis, grain size, weight and grain yield. Two-dimensional differential in-gel electrophoresis identified 40 differentially expressed protein (DEP) spots in waxy and non-waxy wheats, which belonged mainly to starch synthase (SS) I, SS IIa and granule-bound SS I. Most DEPs involved in amylopectin synthesis showed a similar expression pattern during grain development, suggesting relatively independent amylose and amylopectin synthesis pathways. Phosphoproteome analysis of starch granule-binding proteins, using TiO2 microcolumns and LC-MS/MS, showed that the total number of phosphoproteins and their phosphorylation levels in ND5181 were significantly higher than in SN119, but proteins controlling amylopectin synthesis had similar phosphorylation levels. Our results revealed the lack of amylose did not affect the expression and phosphorylation of the starch granule-binding proteins involved in amylopectin biosynthesis.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:35:51.051.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	ming zhang
SpeciesList	scientific name: Triticum aestivum (Wheat); NCBI TaxID: 4565;
ModificationList	phosphorylated residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2013-12-16 02:17:00	ID requested
1	2017-01-17 00:38:25	announced
2	2019-06-04 06:47:57	announced	Updated publication reference for PubMed record(s): 25065648, 27604546.
⏵ 3	2024-10-22 04:35:58	announced	2024-10-22: Updated project metadata.

10.1038/srep33111;

10.1016/j.jprot.2014.07.010;

Chen GX, Zhou JW, Liu YL, Lu XB, Han CX, Zhang WY, Xu YH, Yan YM, Biosynthesis and Regulation of Wheat Amylose and Amylopectin from Proteomic and Phosphoproteomic Characterization of Granule-binding Proteins. Sci Rep, 6():33111(2016) [pubmed]

Zhang M, Lv D, Ge P, Bian Y, Chen G, Zhu G, Li X, Yan Y, Phosphoproteome analysis reveals new drought response and defense mechanisms of seedling leaves in bread wheat (Triticum aestivum L.). J Proteomics, 109():290-308(2014) [pubmed]

curator keyword: Biological

submitter keyword: bread wheat

drought stress

phosphoproteome

phosphorylation status

ming zhang
contact affiliation	school of life sciences
contact email	zhangming5152007@163.com
lab head
ming zhang
contact affiliation	school of life sciences
contact email	zhangming5152007@163.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD000646
     1. Label: PRIDE project
     2. Name: Wheat leaf phosphoproteome under drought stress