PXD000642

PXD000642 is an original dataset announced via ProteomeXchange.

Title	Quantitative proteomics of extracellular vesicles released from human primary macrophages upon beta/glucan stimulation
Description	Fungal infections (mycoses) are common diseases of varying severity that cause problems especially to immunologically compromised people. During stimulatory conditions of infection and colonization, besides intensive intracellular response, human cells actively communicate on the intercellular level by secreting proteins with several mechanisms. The main immunostimulatory components of fungi are beta-glucans, fungal cell-wall components. Here, we have used high-throughput quantitative proteomics combined with bioinformatics to characterize and quantify vesicle-mediated protein release from beta-glucan-stimulated human primary macrophages. We show that β-glucan stimulation induces vesicle-mediated protein secretion. Proteomic study identified 540 distinct proteins from the vesicles including several receptors which have not been identified from vesicles before. Bioinformatics analysis strongly suggests a significant role for these proteins in transfer of biological information. Our current data provides an important resource on the protein composition of extracellular vesicles released from human macrophages upon fungal infection.
HostingRepository	PRIDE
AnnounceDate	2014-07-25
AnnouncementXML	Submission_2014-07-25_01:50:28.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tuula Nyman
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iTRAQ4plex-116 reporter+balance reagent acylated residue; methylthiolated residue
Instrument	QSTAR

Revision	Datetime	Status	ChangeLog Entry
0	2013-12-13 01:48:45	ID requested
1	2014-04-15 02:42:07	announced
⏵ 2	2014-07-25 01:50:29	announced	Updated project metadata.

Cypryk W, Ohman T, Eskelinen EL, Matikainen S, Nyman TA, -glucan stimulation. J Proteome Res, 13(5):2468-77(2014) [pubmed]

submitter keyword: Extracellular vesicles, human primary macrophages, LC-MS/MS, iTRAQ-labeling

Tuula Nyman
contact affiliation	Institute of Biotechnology
contact email	tuula.nyman@helsinki.fi
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2014/04/PXD000642

PRIDE project URI

• PRIDE
  1. PXD000642
     1. Label: PRIDE project
     2. Name: Quantitative proteomics of extracellular vesicles released from human primary macrophages upon beta/glucan stimulation