PXD000640

PXD000640 is an original dataset announced via ProteomeXchange.

Title	Analysis of the interactome of RPS19 mutants
Description	Ribosomal protein S19 (RPS19) is a 16-kDa protein found mainly as a component of the ribosomal 40 S subunit. Its mutations are responsible for Diamond Blackfan anemia, a congenital disease characterized by defective erythroid progenitor maturation. To clarify the function of RPS19, we compare the interactome of RPS19 wilde type and their two mutant proteins: RPS19-R62W and RPS19- R101H. Mutations R62W and R101H impair RPS19 ability to associate with the ribosome. We found striking differences between the interactomes of wild type and mutant RPS19 proteins. In particular, mutations abolish interactions with proteins having splicing, translational and helicase activity, thus confirming the role of RPS19 in RNA processing/metabolism and translational control.
HostingRepository	PRIDE
AnnounceDate	2014-10-23
AnnouncementXML	Submission_2014-10-23_02:48:46.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Marianna Caterino
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue; 2-pyrrolidone-5-carboxylic acid (Gln)
Instrument	6340 Ion Trap LC/MS

Revision	Datetime	Status	ChangeLog Entry
0	2013-12-12 06:11:06	ID requested
1	2014-08-11 00:33:44	announced
⏵ 2	2014-10-23 02:48:47	announced	Updated project metadata.

Caterino M, Aspesi A, Pavesi E, Imperlini E, Pagnozzi D, Ingenito L, Santoro C, Dianzani I, Ruoppolo M, Analysis of the interactome of ribosomal protein S19 mutants. Proteomics, 14(20):2286-96(2014) [pubmed]

curator keyword: Biomedical

submitter keyword: Diamond-Blackfan anemia

protein-protein interaction in ribosome

Margherita Ruoppolo
contact affiliation	Dipartimento di Medicina Molecolare e Biotecnologie Mediche, Universita di Napoli Federico II, Napoli, Italy
contact email	margherita.ruoppolo@unina.it
lab head
Marianna Caterino
contact affiliation	Molecular Diagnostic Laboratory
contact email	marianna.caterino@unina.it
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2014/08/PXD000640

PRIDE project URI

• PRIDE
  1. PXD000640
     1. Label: PRIDE project
     2. Name: Analysis of the interactome of RPS19 mutants