PXD000636

PXD000636 is an original dataset announced via ProteomeXchange.

Title	A conserved AAA+ ATPase network regulates endoplasmic reticulum stress-induced transcription
Description	In C. elegans cdc-48.2(-/-) mutant animals, ER stress-mediated expression of the UPR ckb-2::GFP reporter transgene is abolished. Here, we have used this phenotype in a genome-wide RNAi screen to identify genes involved in the CDC-48.2 mediated ER stress transcription. Combined with a comparative proteomic analysis, this approach has allowed us to identify the AAA+ ATPase RUVB-2 as a novel regulator of the ER stress response and a CDC-48 degradation target.
HostingRepository	PRIDE
AnnounceDate	2015-09-30
AnnouncementXML	Submission_2015-09-30_04:07:19.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD000636
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Dupuy Jean-William
SpeciesList	scientific name: Caenorhabditis elegans; NCBI TaxID: 6239;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	instrument model: LTQ Orbitrap XL; LTQ Orbitrap XL

Revision	Datetime	Status	ChangeLog Entry
0	2013-12-11 14:41:31	ID requested
⏵ 1	2015-09-30 04:07:21	announced

Marza E, Taouji S, Barroso K, Raymond AA, Guignard L, Bonneu M, Pallares-Lupon N, Dupuy JW, Fernandez-Zapico ME, Rosenbaum J, Palladino F, Dupuy D, Chevet E, Genome-wide screen identifies a novel p97/CDC-48-dependent pathway regulating ER-stress-induced gene transcription. EMBO Rep, 16(3):332-40(2015) [pubmed]

submitter keyword: C. elegans, RNAi screen, Proteomic screen, Endoplasmic Reticulum stress, CDC-48, VCP, p97, RUVB-2, Reptin

Chevet Eric
contact affiliation	Team "Endoplasmic Reticulum stress and cancer", INSERM, UMR1053, Bordeaux, France
contact email	eric.chevet@inserm.fr
lab head
Dupuy Jean-William
contact affiliation	University of Bordeaux
contact email	jean-william.dupuy@u-bordeaux.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD000636
     1. Label: PRIDE project
     2. Name: A conserved AAA+ ATPase network regulates endoplasmic reticulum stress-induced transcription