PXD000634

PXD000634 is an original dataset announced via ProteomeXchange.

Title	Low Molecular Weight Allergens from English Walnut (Juglans Regia)
Description	Low molecular weight (LMW) proteins were purified from English walnuts (J. regia cv. Chandler). The LMW proteins were separated by anion exchange chromatography. The anion exchange fractions were analyzed by SDS-PAGE, and protein bands were excised. In gel trypsin digestion was conducted (following reduction with DTT and alkylation with iodoacetamide). Peptides were separated by RP-HPLC and analyzed with an LTQ Orbitrap XL. Peptide and protein identifications were obtained with Mascot.
HostingRepository	PRIDE
AnnounceDate	2019-11-28
AnnouncementXML	Submission_2019-11-28_04:40:50.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD000634
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Melanie Downs
SpeciesList	scientific name: Juglans regia (English walnut); NCBI TaxID: 51240;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	instrument model; LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2013-12-11 07:09:41	ID requested
1	2019-11-28 02:01:33	announced
⏵ 2	2019-11-28 04:40:52	announced	2019-11-28: Updated project metadata.

Downs ML, Semic-Jusufagic A, Simpson A, Bartra J, Fernandez-Rivas M, Rigby NM, Taylor SL, Baumert JL, Mills EN, Characterization of low molecular weight allergens from English walnut (Juglans regia). J Agric Food Chem, 62(48):11767-75(2014) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: Food allergen, Walnut, Vicilin, Tree nut

E.N. Clare Mills
contact affiliation	143 Food Industry Complex, Food Allergy Research and Resource Program, Department of Food Science and Technology, University of Nebraska-Lincoln , Lincoln, Nebraska 68583-0919, United States
contact email	clare.mills@manchester.ac.uk
lab head
Melanie Downs
contact affiliation	Food Allergy Research & Resource Program, Department of Food Science and Technology, University of Nebraska-Lincoln
contact email	mdowns2@unl.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/11/PXD000634

PRIDE project URI

• PRIDE
  1. PXD000634
     1. Label: PRIDE project
     2. Name: Low Molecular Weight Allergens from English Walnut (Juglans Regia)