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PXD000632

PXD000632 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleFLAG-affinity pulldown experiments with sll1214 and sll1874 from Synechocystis
DescriptionPutative subunits of the Mg-protoporphyrin monomethylester oxidative cyclase from Synechocystis PCC 6803, sll1214 and sll1874 were expressed with N-terminal 3xFLAG-tags in separate transformants.. Solubilised thylakoid membranes from these 2 transformants were incubated with anti-FLAG agarose and after extensive washing, the FLAG-tagged proteins, together with putative interaction partners were desorbed with FLAG peptide. The eluates were denatured, reduced, alkylated and digested with trypsin. The resulting peptides were cleaned by cation exchange and desalted, then analysed by nanoLC-MS/MS. The FLAG pulldown experiment was also carried out with a thylakoid membrane preparation from wild-type with no FLAG-tagged proteins as a control. Protein identification was by database searching with Mascot.
HostingRepositoryPRIDE
AnnounceDate2015-08-05
AnnouncementXMLSubmission_2015-08-05_04:20:16.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD000632
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterPhilip Jackson
SpeciesList scientific name: Synechocystis sp. (strain PCC 6803 / Kazusa); NCBI TaxID: 1111708;
ModificationListdeamidated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrumentinstrument model: Maxis UHR-TOF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02013-12-10 04:02:12ID requested
12015-08-05 04:20:17announced
Publication List
Hollingshead S, Kopecn, รก J, Jackson PJ, Canniffe DP, Davison PA, Dickman MJ, Sobotka R, Hunter CN, Conserved chloroplast open-reading frame ycf54 is required for activity of the magnesium protoporphyrin monomethylester oxidative cyclase in Synechocystis PCC 6803. J Biol Chem, 287(33):27823-33(2012) [pubmed]
Keyword List
submitter keyword: Synechocystis PCC 6803, Mg-protoporphyrin monomethylester oxidative cyclase, sll1214, sll1874, Ycf54, FLAG-tag, Maxis UHR-TOF
Contact List
Philip Jackson
contact affiliationDepartment of Chemical and Biological Engineering
contact emailp.j.jackson@sheffield.ac.uk
lab head
Philip Jackson
contact affiliationDepartment of Chemical and Biological Engineering
contact emailp.j.jackson@sheffield.ac.uk
dataset submitter
Full Dataset Link List
Dataset FTP location
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