PXD000632 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | FLAG-affinity pulldown experiments with sll1214 and sll1874 from Synechocystis |
Description | Putative subunits of the Mg-protoporphyrin monomethylester oxidative cyclase from Synechocystis PCC 6803, sll1214 and sll1874 were expressed with N-terminal 3xFLAG-tags in separate transformants.. Solubilised thylakoid membranes from these 2 transformants were incubated with anti-FLAG agarose and after extensive washing, the FLAG-tagged proteins, together with putative interaction partners were desorbed with FLAG peptide. The eluates were denatured, reduced, alkylated and digested with trypsin. The resulting peptides were cleaned by cation exchange and desalted, then analysed by nanoLC-MS/MS. The FLAG pulldown experiment was also carried out with a thylakoid membrane preparation from wild-type with no FLAG-tagged proteins as a control. Protein identification was by database searching with Mascot. |
HostingRepository | PRIDE |
AnnounceDate | 2015-08-05 |
AnnouncementXML | Submission_2015-08-05_04:20:16.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD000632 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Philip Jackson |
SpeciesList | scientific name: Synechocystis sp. (strain PCC 6803 / Kazusa); NCBI TaxID: 1111708; |
ModificationList | deamidated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | instrument model: Maxis UHR-TOF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2013-12-10 04:02:12 | ID requested | |
⏵ 1 | 2015-08-05 04:20:17 | announced | |
Publication List
Hollingshead S, Kopecn, รก J, Jackson PJ, Canniffe DP, Davison PA, Dickman MJ, Sobotka R, Hunter CN, Conserved chloroplast open-reading frame ycf54 is required for activity of the magnesium protoporphyrin monomethylester oxidative cyclase in Synechocystis PCC 6803. J Biol Chem, 287(33):27823-33(2012) [pubmed] |
Keyword List
submitter keyword: Synechocystis PCC 6803, Mg-protoporphyrin monomethylester oxidative cyclase, sll1214, sll1874, Ycf54, FLAG-tag, Maxis UHR-TOF |
Contact List
Philip Jackson |
contact affiliation | Department of Chemical and Biological Engineering |
contact email | p.j.jackson@sheffield.ac.uk |
lab head | |
Philip Jackson |
contact affiliation | Department of Chemical and Biological Engineering |
contact email | p.j.jackson@sheffield.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD000632
- Label: PRIDE project
- Name: FLAG-affinity pulldown experiments with sll1214 and sll1874 from Synechocystis