PXD000627

PXD000627 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of Pichia pastoris GS115
Description	Pichia pastoris GS115 was cultured in YPD medium and protein were extracted and separated on SDS-PAGE followed by in-gel trypsin digestion. Protein were also digested in-solution using trypsin and fractionated using SCX and bRPLC. A total of 101 peptide fractions were analyzed on LTQ-Orbitrap Velos mass spectrometer. The raw data was searched using Mascot, Sequest and MS Amanda search algorithms against P. pastorisGS115 NCBI RefSeq database through Proteome Discoverer software suite.
HostingRepository	PRIDE
AnnounceDate	2014-11-18
AnnouncementXML	Submission_2014-11-18_08:28:04.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Akhilesh Pandey
SpeciesList	scientific name: Komagataella pastoris (strain GS115 / ATCC 20864) (Yeast) (Pichia pastoris); NCBI TaxID: 644223;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2013-12-08 14:26:38	ID requested
1	2014-11-18 00:46:53	announced
⏵ 2	2014-11-18 08:28:05	announced	Updated publication details.

Renuse S, Madugundu AK, Kumar P, Nair BG, Gowda H, Prasad TS, Pandey A, Proteomic analysis and genome annotation of Pichia pastoris, a recombinant protein expression host. Proteomics, 14(23-24):2769-79(2014) [pubmed]

curator keyword: Biological

submitter keyword: Pichia, LC-MS/MS

Akhilesh Pandey
contact affiliation	Johns Hopkins University
contact email	pandey@jhmi.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2014/11/PXD000627

PRIDE project URI

• PRIDE
  1. PXD000627
     1. Label: PRIDE project
     2. Name: Proteomic analysis of Pichia pastoris GS115