PXD000620

PXD000620 is an original dataset announced via ProteomeXchange.

Title	Cervico-Vaginal mucus proteins of buffalo
Description	Cervico-vaginal fluid (CVF) plays significant roles in coitus, sperm transport and implantation. It is believed to be a good non-invasive biomarker for various diagnostic purposes. In this study, a comprehensive proteomic analysis of buffalo CVF was performed during the estrous cycle in order to document the protein expressions, utilizing SDS-PAGE, mass spectrometry, and immunoblot.Xcalibur software (version 2.0. SR1) was used for analyzing the mass spectrometry data. Product ion scans gathered from tandem mass spectrometry were involved in the database search software SEQUEST (TURBO) (http://sjsupport.thermofinnigan.com/project/product_support/xcal_layered_apps_turbosequest.htm). Peak lists were created from the products in the scan data (threshold set to 10,000) and these were searched against the mammalian protein sequence database from NCBI (http://www.ncbi.nlm.nih.gov/). Modification of cysteine by carboxymethylation and methionine by oxidation modifications was allowed. The mass tolerance for the precursor peptide ions was set to 3.5 and the fragment ion tolerance was set to 1. Singly charged, doubly charged and triply charged peptides have higher cross-correlation score viz., 1.9, 2.2 and 3.75, respectively, which give high confidence in terms of protein identification. More than two unique peptides for each protein were taken for confirmation of the protein present in the sample.
HostingRepository	PRIDE
AnnounceDate	2014-07-24
AnnouncementXML	Submission_2014-07-24_05:16:24.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Subramanian Muthukumar
SpeciesList	scientific name: Bubalus bubalis; NCBI TaxID: 89462;
ModificationList	iodoacetic acid derivatized residue; monohydroxylated residue
Instrument	Thermo Electron instrument model

Revision	Datetime	Status	ChangeLog Entry
0	2013-12-05 02:02:20	ID requested
1	2014-03-17 04:18:27	announced
⏵ 2	2014-07-24 05:16:25	announced	Updated project metadata.

Muthukumar S, Rajkumar R, Karthikeyan K, Liao CC, Singh D, Akbarsha MA, Archunan G, Buffalo cervico-vaginal fluid proteomics with special reference to estrous cycle: heat shock protein (HSP)-70 appears to be an estrus indicator. Biol Reprod, 90(5):97(2014) [pubmed]

curator keyword: Biological

submitter keyword: buffalo, estrous cycle, cervico-vaginal mucus, SDS-PAGE, LC-MSMS

G Archunan
contact affiliation	Deparment of Animal Science, Bharathidasan University, Tiruchirappalli, Tamilnadu, India
contact email	archunan@bdu.ac.in
lab head
Subramanian Muthukumar
contact affiliation	Centre for Pheromone Technology, Department of Animal Science, Bharathidasan University, Tiruchirappalli, India
contact email	sasmuthu@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2014/03/PXD000620

PRIDE project URI

• PRIDE
  1. PXD000620
     1. Label: PRIDE project
     2. Name: Cervico-Vaginal mucus proteins of buffalo