PXD000618

PXD000618 is an original dataset announced via ProteomeXchange.

Title	Characterization of the proteome of human blood platelet sercretory granules using gas-phase fractionation (GPF)
Description	We performed an extensive qualitative characterization of the platelet granule proteome using subcellular fractionation followed by mass spectrometry analysis and functional annotation. Platelets were isolated from whole blood by centrifugation and their level of purity was tested by microscopic inspection, western blot and Leucocount kit. Subcellular fractionation based on sucrose gradient was used to enrich sample in secretory granules. Then sample quality was assessed by western blot and electronic microscopy, prior proteomic study. Samples were trypsin digested and analyzed using an Orbitrap Velos in a gas-phase fractionation mode. The m/z ranges for the selection of precursor ions were 400-521, 516-690, 685-986 and 963-2000 Thomsons. The 2 technical replicates were analyzed by a GPF series of injections. Protein identification was performed using the EasyProt resource. The module EasyProtConv was used to generate the MGF file from the raw data. The peaklist files were searched against the UniProt database (2011_02 of 08-Feb-2011) using EasyProt. Eight-hundred-and-twenty-seven proteins were identified, most of them being associated to granules and to the granule’s secretory machinery.
HostingRepository	PRIDE
AnnounceDate	2014-07-24
AnnouncementXML	Submission_2014-07-24_03:48:25.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Pierre Fontana
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2013-12-04 10:22:48	ID requested
1	2014-03-06 02:41:56	announced
⏵ 2	2014-07-24 03:48:25	announced	Updated project metadata.

Zufferey A, Schvartz D, Nolli S, Reny JL, Sanchez JC, Fontana P, Characterization of the platelet granule proteome: evidence of the presence of MHC1 in alpha-granules. J Proteomics, 101():130-40(2014) [pubmed]

curator keyword: Biological, Technical

submitter keyword: Platelet, secretory granules, GPF4

Pierre Fontana
contact affiliation	Angiology and Heamostasis, University Hospitals of Geneva
contact email	pierre.fontana@hcuge.ch
lab head
Pierre Fontana
contact affiliation	Angiology and Heamostasis
contact email	pierre.fontana@hcuge.ch
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD000618
     1. Label: PRIDE project
     2. Name: Characterization of the proteome of human blood platelet sercretory granules using gas-phase fractionation (GPF)