PXD000606

PXD000606 is an original dataset announced via ProteomeXchange.

Title	Proteomic Analysis of Protein Methylation in the Yeast Saccharomyces cerevisiae
Description	Protein methylation catalyzed by SAM-dependent methyltransferase represents a major PTM involved in important biological processes. Because methylation can occur on nitrogen, oxygen and sulfur centers and multiple methylation states exist on the nitrogen centers, methylproteome remains poorly documented. Here we present the methylation by isotope labeled SAM (MILS) strategy for a highly-confident analysis of the methylproteome of the SAM-auxotrophic Saccharomyces cerevisiae based on the online multidimensional μHPLC/MS/MS technology. We identified 117 methylated proteins, containing 182 methylation events associated with 174 methylation sites. About 90% of these methylation events were previously unknown. Our results indicated, 1) over 6% of the yeast proteome are methylated, 2) the amino acid residue preference of protein methylation follows the order Lys >> Arg > Asp > Glu ≈ Gln ≈ Asp > His > Cys, 3) the methylation state on nitrogen center is largely exclusive, and 4) the methylated proteins are located mainly in nucleus/ribosome associated with translation/transcription and DNA/RNA processing. Our dataset is the most comprehensive methylproteome known-to-date of all living organisms, and should significantly contribute to the field of protein methylation and related research.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:59:33.621.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	KEYUN WANG
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	monomethylated residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2013-11-27 07:00:02	ID requested
1	2020-01-27 01:45:11	announced
⏵ 2	2024-10-22 04:59:34	announced	2024-10-22: Updated project metadata.

10.1016/j.jprot.2014.07.032;

Wang K, Zhou YJ, Liu H, Cheng K, Mao J, Wang F, Liu W, Ye M, Zhao ZK, Zou H, Proteomic analysis of protein methylation in the yeast Saccharomyces cerevisiae. J Proteomics, 114():226-33(2015) [pubmed]

curator keyword: Biological

submitter keyword: LC/MS/MS, Methylation, Proteomics,Saccharomyces cerevisiae

Hanfa Zou
contact affiliation	Division of Biotechnology, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China
contact email	hanfazou@dicp.ac.cn
lab head
KEYUN WANG
contact affiliation	Division of Biotechnology
contact email	wkyhnu1988@126.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/01/PXD000606

PRIDE project URI

• PRIDE
  1. PXD000606
     1. Label: PRIDE project
     2. Name: Proteomic Analysis of Protein Methylation in the Yeast Saccharomyces cerevisiae