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PXD000591

PXD000591 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleHuman Adenovirus 5 (HAdV5), LC-MSMS
DescriptionUsing high-resolution mass spectrometry (MS)-based proteomics in combination with multiple protease digestion, we profiled, with on average 90% sequence coverage, all 13 viral proteins present in an human adenovirus (HAdV) vector. This in-depth profile provided multiple peptide-based evidence on intrinsic protease activity affecting several HAdV proteins. Next, the generated peptide library was used to develop a targeted proteomics method using selected reaction monitoring (SRM) aimed at quantitative profiling of the stoichiometry of all 13 proteins present in the HAdV. We also used this method to probe the release of specific virus proteins initiated by thermal stimulation, mimicking the early stage of HAdV disassembly during entry into host cells. We confirmed the copy numbers of the most well characterized viral capsid components and established the copy numbers for proteins whose stoichiometry has so far not been accurately defined. We also found that heating HAdV induces the complete release of the penton base and fiber proteins as well as a substantial release of protein VIII and VI. For these latter proteins, maturational proteolysis by the adenoviral protease (AVP) leads to the differential release of fragments with certain peptides being fully released and others largely retained in the AdV particles. This information is likely to be beneficial for the ongoing interpretation of high resolution cryoEM and X-ray electron density maps.
HostingRepositoryPRIDE
AnnounceDate2014-07-24
AnnouncementXMLSubmission_2014-07-24_05:20:27.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMarco Benevento
SpeciesList scientific name: Human adenovirus C serotype 5 (HAdV-5) (Human adenovirus 5); NCBI TaxID: ADE05;
ModificationListacetylated residue
InstrumentLTQ Orbitrap; Q Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02013-11-21 08:02:23ID requested
12014-06-09 02:38:44announced
22014-07-24 05:20:28announcedUpdated project metadata.
Publication List
Benevento M, Di Palma S, Snijder J, Moyer CL, Reddy VS, Nemerow GR, Heck AJR, Adenovirus composition, proteolysis, and disassembly studied by in-depth qualitative and quantitative proteomics. J Biol Chem, 289(16):11421-11430(2014) [pubmed]
Keyword List
ProteomeXchange project tag: PRIME-XS Project
curator keyword: Biomedical
submitter keyword: Adenovirus, quantification, proteomics
Contact List
Albert J.R. Heck
contact affiliationBiomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Science, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands; Netherlands Proteomics Centre, Padualaan 8, 3584 CH Utrecht, The Netherlands
contact emaila.j.r.heck@uu.nl
lab head
Marco Benevento
contact affiliationFaculty of Sciences
contact emailm.benevento@uu.nl
dataset submitter
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Dataset FTP location
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