PXD000569

PXD000569 is an original dataset announced via ProteomeXchange.

Title	S-nitrosylation in Chlamydomonas reinhardtii
Description	Protein S-nitrosylation, a post-translational modification consisting in the covalent binding of nitric oxide (NO) to a cysteine thiol moiety, plays a major role in cell signaling and is recognized to be involved in numerous physiological processes and diseases in mammals. The importance of nitrosylation in photosynthetic eukaryotes has been less studied. The aim of this study was to expand our knowledge on protein nitrosylation by performing a large scale proteomic analysis of proteins undergoing nitrosylation in vivo in Chlamydomonas reinhardtii cells under nitrosative stress. Using two complementary proteomic approaches, 492 nitrosylated proteins were identified. They participate in a wide range of biological processes and pathways including photosynthesis, carbohydrate metabolism, amino acid metabolism, translation, protein folding or degradation, cell motility and stress. Several proteins were confirmed in vitro by western blot, site-directed mutagenesis and activity measurements. Moreover, 392 sites of nitrosylation were also identified. These results strongly suggest that S-nitrosylation could constitute a major mechanism of regulation in C. reinhardtii under nitrosative stress conditions. This study constitutes the largest proteomic analysis of protein nitrosylation reported to date. The identification of 381 previously unrecognized targets of nitrosylation further extends our knowledge on the importance of this post-translational modification in photosynthetic eukaryotes.
HostingRepository	PRIDE
AnnounceDate	2014-07-25
AnnouncementXML	Submission_2014-07-25_01:45:45.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Christophe MARCHAND
SpeciesList	scientific name: Chlamydomonas reinhardtii (Chlamydomonas smithii); NCBI TaxID: 3055;
ModificationList	N-ethylmaleimide derivatized cysteine; acetylated residue; iodoacetamide derivatized residue; L-methionine sulfoxide
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2013-11-05 05:03:19	ID requested
1	2013-12-19 03:57:52	announced
1	2013-12-19 04:04:21	announced
⏵ 2	2014-07-25 01:45:46	announced	Updated project metadata.

Morisse S, Zaffagnini M, Gao XH, Lemaire SD, Marchand CH, Insight into protein S-nitrosylation in Chlamydomonas reinhardtii. Antioxid Redox Signal, 21(9):1271-84(2014) [pubmed]

submitter keyword: nitrosylation

nitric oxide

nitrosoglutathione

Chlamydomonas reinhardtii

proteomics

biotin-switch

SNOSID

Christophe MARCHAND
contact affiliation	Laboratoire de Biologie Moléculaire et Cellulaire des Eucaryotes FRE3354 - CNRS/UPMC - IBPC
contact email	christophe.marchand@ibpc.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD000569
     1. Label: PRIDE project
     2. Name: S-nitrosylation in Chlamydomonas reinhardtii