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PXD000564

PXD000564 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleLC-MS/MS analysis of visceral and subcutaneous adipose tissue proteomes in young goats with focus on innate immunity and inflammation related proteins
DescriptionThe aim of this study was to undertake an in-depth and comparative study of the protein expression patterns of subcutaneous and visceral adipose tissues in goats, combined to an mRNA expression study of proteins involved in immune and inflammatory response. Samples were obtained from four healthy goat-kids, Alpine breed, naturally reared by their mothers. Animals were slaughtered at the age of 30 days, during routinely slaughtering procedures, and four different adipose tissues were collected from each animal. Subcutaneous fat was taken from sternum and base of the tail; visceral fat was taken from perirenal and omental depots. The four adipose tissues deposits were selected due to their frequent use in experimental studies on fat tissue. Liver samples were also collected, and used as reference samples. Tissue samples were snap frozen in liquid nitrogen and stored at –80 degrees C until analysis. The 20 runs included in this dataset are from the 4 animals x 5 tissues (sternum, base of the tail, perirenal area, omentum and liver). A 2-D label free LC-MS/MS approach followed by cluster analysis was used for comparing the subcutaneous and visceral fat tissue proteomes. Each sample was analysed in ~12 SCX fractions which correspond to the WIFF files included in each RAW_GOAT0xx.zip files.
HostingRepositoryPRIDE
AnnounceDate2015-07-20
AnnouncementXMLSubmission_2015-07-20_06:29:42.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterLaura Restelli
SpeciesList scientific name: Capra hircus (Goat); NCBI TaxID: 9925;
ModificationListmonohydroxylated residue
InstrumentQSTAR
Dataset History
RevisionDatetimeStatusChangeLog Entry
02013-11-04 05:51:14ID requested
12015-07-20 06:29:43announced
Publication List
Restelli L, Codrea MC, Savoini G, Ceciliani F, Bendixen E, LC-MS/MS analysis of visceral and subcutaneous adipose tissue proteomes in young goats with focus on innate immunity and inflammation related proteins. J Proteomics, 108():295-305(2014) [pubmed]
Keyword List
submitter keyword: Subcutaneous adipose tissue
visceral adipose tissue
LC-MS/MS
adipokines
goat
Contact List
Laura Restelli
contact affiliationLaura Restelli, Dipartimento di Scienze Veterinarie per la Salute, la Produzione Animale e la Sicurezza Alimentare – Università degli Studi di Milano, Via Celoria 10, 20133 Milan, Italy. Phone: +39 02 50318100
contact emaillaura.restelli1@hotmail.it
lab head
Laura Restelli
contact affiliationDipartimento di Scienze Veterinarie per la Salute, la Produzione Animale e la Sicurezza Alimentare
contact emaillaura.restelli1@hotmail.it
dataset submitter
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Dataset FTP location
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