PXD000564 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | LC-MS/MS analysis of visceral and subcutaneous adipose tissue proteomes in young goats with focus on innate immunity and inflammation related proteins |
Description | The aim of this study was to undertake an in-depth and comparative study of the protein expression patterns of subcutaneous and visceral adipose tissues in goats, combined to an mRNA expression study of proteins involved in immune and inflammatory response. Samples were obtained from four healthy goat-kids, Alpine breed, naturally reared by their mothers. Animals were slaughtered at the age of 30 days, during routinely slaughtering procedures, and four different adipose tissues were collected from each animal. Subcutaneous fat was taken from sternum and base of the tail; visceral fat was taken from perirenal and omental depots. The four adipose tissues deposits were selected due to their frequent use in experimental studies on fat tissue. Liver samples were also collected, and used as reference samples. Tissue samples were snap frozen in liquid nitrogen and stored at –80 degrees C until analysis. The 20 runs included in this dataset are from the 4 animals x 5 tissues (sternum, base of the tail, perirenal area, omentum and liver). A 2-D label free LC-MS/MS approach followed by cluster analysis was used for comparing the subcutaneous and visceral fat tissue proteomes. Each sample was analysed in ~12 SCX fractions which correspond to the WIFF files included in each RAW_GOAT0xx.zip files. |
HostingRepository | PRIDE |
AnnounceDate | 2015-07-20 |
AnnouncementXML | Submission_2015-07-20_06:29:42.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Laura Restelli |
SpeciesList | scientific name: Capra hircus (Goat); NCBI TaxID: 9925; |
ModificationList | monohydroxylated residue |
Instrument | QSTAR |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2013-11-04 05:51:14 | ID requested | |
⏵ 1 | 2015-07-20 06:29:43 | announced | |
Publication List
Restelli L, Codrea MC, Savoini G, Ceciliani F, Bendixen E, LC-MS/MS analysis of visceral and subcutaneous adipose tissue proteomes in young goats with focus on innate immunity and inflammation related proteins. J Proteomics, 108():295-305(2014) [pubmed] |
Keyword List
submitter keyword: Subcutaneous adipose tissue |
visceral adipose tissue |
LC-MS/MS |
adipokines |
goat |
Contact List
Laura Restelli |
contact affiliation | Laura Restelli, Dipartimento di Scienze Veterinarie per la Salute, la Produzione Animale e la Sicurezza Alimentare – Università degli Studi di Milano, Via Celoria 10, 20133 Milan, Italy. Phone: +39 02 50318100 |
contact email | laura.restelli1@hotmail.it |
lab head | |
Laura Restelli |
contact affiliation | Dipartimento di Scienze Veterinarie per la Salute, la Produzione Animale e la Sicurezza Alimentare |
contact email | laura.restelli1@hotmail.it |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/07/PXD000564 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD000564
- Label: PRIDE project
- Name: LC-MS/MS analysis of visceral and subcutaneous adipose tissue proteomes in young goats with focus on innate immunity and inflammation related proteins