PXD000558

PXD000558 is an original dataset announced via ProteomeXchange.

Title	Phosphoproteomics of MuSK Signaling
Description	The development of the neuromuscular synapse depends on signaling processes which involve protein phosphorylation as a crucial regulatory event. The receptor tyrosine kinase MuSK is the key signaling molecule at the neuromuscular synapse whose activity is required for the formation of a mature and functional neuromuscular synapse. However, the signaling cascade downstream of MuSK and the regulation of the different components is still poorly understood. Here we present data from a quantitative phosphoproteomics approach to study MuSK-dependent processes. Muscle cells were stimulated with the heparansulfate proteoglycan agrin, which activates MuSK and downstream signaling events. To get an insight into the signaling dynamics we used three different time points (unstimulated, 15 min, 60 min and 4 hrs).
HostingRepository	PRIDE
AnnounceDate	2014-07-24
AnnouncementXML	Submission_2014-07-24_05:19:59.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD000558
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Gerhard Duernberger
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; phosphorylated residue; iTRAQ4plex-116 reporter+balance reagent acylated residue; methylthiolated residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2013-10-31 06:24:00	ID requested
1	2014-06-05 09:20:09	announced
2	2014-06-06 02:01:18	announced	Updated publication reference for PubMed record(s): 24899341.
⏵ 3	2014-07-24 05:20:00	announced	Updated project metadata.

D, ü, rnberger G, Camurdanoglu BZ, Tomschik M, Schutzbier M, Roitinger E, Hudecz O, Mechtler K, Herbst R, Global analysis of muscle-specific kinase signaling by quantitative phosphoproteomics. Mol Cell Proteomics, 13(8):1993-2003(2014) [pubmed]

curator keyword: Biomedical, Biological

submitter keyword: MuSK, mouse, phospho, neuromuscular synapse

Karl Mechtler
contact affiliation	IMBA, Institute of Molecular Biotechnology of the Austrian Academy of Sciences, Vienna, Austria; IMP, Institute for Molecular Pathology, Vienna, Austria
contact email	karl.mechtler@imp.ac.at
lab head
Gerhard Duernberger
contact affiliation	GMI/IMP/IMBA
contact email	gerhard.duernberger@imp.ac.at
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD000558
     1. Label: PRIDE project
     2. Name: Phosphoproteomics of MuSK Signaling