PXD000552

PXD000552 is an original dataset announced via ProteomeXchange.

Title	Deep and precise quantification of the mouse synaptosomal proteome reveals substantial remodeling during postnatal maturation
Description	In order to understand the molecular underpinnings of the functional and morphological heterogeneity of synapses and their regulation during development it is essential to identify the set of proteins that form these structures. Here, we prepared cortical synaptosomal fractions from juvenile and adult mice, processed them using a multidimentional protein identification technology approach, applied iTRAQ labelling and used mass spectroscopy to identify a comprehensive set of synaptic proteins and to quantitatively analyze changes in protein expression during development. We obtained a dataset tracking expression levels of 3424 proteins during development. The inclusion of biological replicates in a single mass spectrometry analysis demonstrated a high accuracy of our method. A gene ontology analysis confirmed the synaptic nature of a large fraction of identified proteins. Furthermore, developmental regulation of eight proteins identified in our analysis was confirmed independently using Western blotting. In summary, we provide a comprehensive dataset describing the developmental regulation of synaptosomal proteins, which serves as an essential foundation for the investigation of synaptic function and maturation.
HostingRepository	PRIDE
AnnounceDate	2014-10-23
AnnouncementXML	Submission_2014-10-23_02:43:31.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Michael Schutzbier
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	iTRAQ4plex-116 reporter+balance reagent acylated residue; methylthiolated residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2013-10-30 02:20:06	ID requested
1	2014-08-28 04:36:39	announced
2	2014-08-29 00:30:38	announced	Updated project metadata.
⏵ 3	2014-10-23 02:43:32	announced	Updated project metadata.

Moczulska KE, Pichler P, Schutzbier M, Schleiffer A, Rumpel S, Mechtler K, Deep and precise quantification of the mouse synaptosomal proteome reveals substantial remodeling during postnatal maturation. J Proteome Res, 13(10):4310-24(2014) [pubmed]

curator keyword: Biological

submitter keyword: synapse proteome ageing

Michael Schutzbier
contact affiliation	Service
contact email	schutzbier@imp.ac.at
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2014/08/PXD000552

PRIDE project URI

• PRIDE
  1. PXD000552
     1. Label: PRIDE project
     2. Name: Deep and precise quantification of the mouse synaptosomal proteome reveals substantial remodeling during postnatal maturation