PXD000545 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Photosynthetic Megacomplex in Synechosystis 6803 |
Description | Phycobilisomes capture light energy and feed into reaction centers in cyanobacteria and red algae. Their ability to capture light across a broad spectral range, distinct from those of the chlorophylls and carotenoids, expand photosynthetic solar energy transformation globally. The energy transfer from phycobilisome to reaction centers has been established for decades, however, remarkably little is known about the structural interface between the phycobilisome and the thylakoids membrane and the reaction centers. Here, we isolated a megacomplex composed of phycobilisome with its energy acceptors, i.e., photosystem I (PSI) and photosystem II (PSII). Functional analysis showed there are highly active oxygen-evolving PSII activity and O2 consumption activity from PSI in the megacomplex. Steady state fluorescence spectroscopy analysis indicated efficient excitation energy transfer from phycobilisome to both photosystems. LC-MS analysis preceded by cross-linking chemistry identified the close association of ApcE and CP43, and of ApcD and PsaA. Mass spectrometry also identified the structural proximity of ApcD and ApcE. Femto-second time resolved fluorescence spectroscopy were employed to study the kinetic energy transfer from phycobilisome to two reaction centers in the megacomplex. |
HostingRepository | PRIDE |
AnnounceDate | 2014-07-25 |
AnnouncementXML | Submission_2014-07-25_01:44:05.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Hao Zhang |
SpeciesList | scientific name: Synechocystis sp. PCC 6803; NCBI TaxID: 1148; |
ModificationList | monohydroxylated residue |
Instrument | LTQ Orbitrap |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2013-10-28 04:32:27 | ID requested | |
1 | 2013-11-29 04:58:40 | announced | |
⏵ 2 | 2014-07-25 01:44:05 | announced | Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Cyanobacteria, Phycobilisomes, LC-MS/MS, Cross-linking, Photosystem I, Photosystem II |
Contact List
Hao Zhang |
contact affiliation | Washington University in St. Louis |
contact email | zhanghao@wustl.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD000545
- Label: PRIDE project
- Name: Photosynthetic Megacomplex in Synechosystis 6803