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PXD000545

PXD000545 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitlePhotosynthetic Megacomplex in Synechosystis 6803
DescriptionPhycobilisomes capture light energy and feed into reaction centers in cyanobacteria and red algae. Their ability to capture light across a broad spectral range, distinct from those of the chlorophylls and carotenoids, expand photosynthetic solar energy transformation globally. The energy transfer from phycobilisome to reaction centers has been established for decades, however, remarkably little is known about the structural interface between the phycobilisome and the thylakoids membrane and the reaction centers. Here, we isolated a megacomplex composed of phycobilisome with its energy acceptors, i.e., photosystem I (PSI) and photosystem II (PSII). Functional analysis showed there are highly active oxygen-evolving PSII activity and O2 consumption activity from PSI in the megacomplex. Steady state fluorescence spectroscopy analysis indicated efficient excitation energy transfer from phycobilisome to both photosystems. LC-MS analysis preceded by cross-linking chemistry identified the close association of ApcE and CP43, and of ApcD and PsaA. Mass spectrometry also identified the structural proximity of ApcD and ApcE. Femto-second time resolved fluorescence spectroscopy were employed to study the kinetic energy transfer from phycobilisome to two reaction centers in the megacomplex.
HostingRepositoryPRIDE
AnnounceDate2014-07-25
AnnouncementXMLSubmission_2014-07-25_01:44:05.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterHao Zhang
SpeciesList scientific name: Synechocystis sp. PCC 6803; NCBI TaxID: 1148;
ModificationListmonohydroxylated residue
InstrumentLTQ Orbitrap
Dataset History
RevisionDatetimeStatusChangeLog Entry
02013-10-28 04:32:27ID requested
12013-11-29 04:58:40announced
22014-07-25 01:44:05announcedUpdated project metadata.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Cyanobacteria, Phycobilisomes, LC-MS/MS, Cross-linking, Photosystem I, Photosystem II
Contact List
Hao Zhang
contact affiliationWashington University in St. Louis
contact emailzhanghao@wustl.edu
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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