PXD000541

PXD000541 is an original dataset announced via ProteomeXchange.

Title	2D-LC/MS/MS analysis of Escherichia coli during exposure an abrupt downshift in water activity
Description	2D-LC/MS/MS analysis was used to examine time-dependent changes in proteome of E. coli O157:H7 strain Sakai upon an abrupt downshift in water activity (aw) (i.e., from aw 0.993 to aw 0.967) at a constant temperature of 35°C. Bacterial cells were harvested before hyperosmotic shift, and 0 (i.e. immediately after the shift), 30, 80, and 310 min after the shift. It also should be noted that these time points were chosen with the aims to characterize the physiology of E. coli during dynamic changes in growth kinetics induced by an abrupt downshift in water activity. Specifically, the samples taken at time 0 and 30 min were obtained during the period in which the loss of cell culturability was observed, whereas the samples at time 80 and 310 min respectively reflected the physiological state of E. coli during the ‘recovery’ period and during growth after the shift.
HostingRepository	PRIDE
AnnounceDate	2014-07-25
AnnouncementXML	Submission_2014-07-25_01:43:42.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Chawalit Kocharunchitt
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	iodoacetamide derivatized residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2013-10-28 02:09:50	ID requested
1	2014-04-14 03:19:53	announced
⏵ 2	2014-07-25 01:43:43	announced	Updated project metadata.

Kocharunchitt C, King T, Gobius K, Bowman JP, Ross T, H7 Sakai during dynamic changes in growth kinetics induced by an abrupt downshift in water activity. PLoS One, 9(3):e90422(2014) [pubmed]

submitter keyword: E. coli, 2D-LC/MS/MS, physiology, stress response, water activity stress

Chawalit Kocharunchitt
contact affiliation	University of Tasmania
contact email	chawalit.kocharunchitt@utas.edu.au
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2014/04/PXD000541

PRIDE project URI

• PRIDE
  1. PXD000541
     1. Label: PRIDE project
     2. Name: 2D-LC/MS/MS analysis of Escherichia coli during exposure an abrupt downshift in water activity