PXD000537

PXD000537 is an original dataset announced via ProteomeXchange.

Title	Dynamic impacts of the inhibition of the molecular chaperone Hsp90 on the T-cell proteome have implications for anti-cancer therapy.
Description	Using an integrated systematic approach to analyse the effects of Hsp90 inhibition in T-cells, we quantified differential changes in the Hsp90-dependent proteome, Hsp90 interactome, and a selection of the transcriptome. Kinetic behaviours in the Hsp90-dependent proteome were assessed using a novel pulse-chase strategy (pulse-chase SILAC, Fierro-Monti et al., accompanying article), detecting effects on both protein stability and synthesis. Global and specific dynamic impacts, including proteostatic responses, are due to direct inhibition of Hsp90 as well as indirect effects. A variety of effects on protein levels and kinetic behaviors are described. Methods : Jurkat cells were treated with DMSO or 1 uM Geldanamycin for 6 or 20h and the proteome was compared to that of untreated cells at both time points. Three biological replicates were prepared and analysed for each condition, one replicate with label inversion. Cell extracts and tryptic digestion were performed according to the FASP protocol. Peptide mixtures were separated into 24 fractions by off-gel isoelectric focusing and analysed by nano-LC-MS/MS on an Orbitrap Velos Instrument. Data were analysed as duplex SILAC using MaxQuant and peptide spectrum matches were filtered at 1% FDR. Output tables were processed further with custom-made perl scripts for further filtering.
HostingRepository	PRIDE
AnnounceDate	2014-08-08
AnnouncementXML	Submission_2014-08-08_00:31:09.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Manfredo Quadroni
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	S-carboxamidomethyl-L-cysteine; 6x(13)C labeled L-lysine; 6x(13)C labeled L-arginine
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2013-10-24 06:00:19	ID requested
1	2013-12-10 05:55:39	announced
1	2013-12-10 06:02:32	announced
2	2014-07-25 01:43:06	announced	Updated project metadata.
⏵ 3	2014-08-08 00:31:10	announced	Updated project metadata.

Fierro-Monti I, Echeverria P, Racle J, Hernandez C, Picard D, Quadroni M, Dynamic impacts of the inhibition of the molecular chaperone Hsp90 on the T-cell proteome have implications for anti-cancer therapy. PLoS One, 8(11):e80425(2013) [pubmed]

curator keyword: Biomedical

submitter keyword: SILAC, T-cells, HSP90

Manfredo Quadroni
contact affiliation	University of Lausanne
contact email	manfredo.quadroni@unil.ch
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD000537
     1. Label: PRIDE project
     2. Name: Dynamic impacts of the inhibition of the molecular chaperone Hsp90 on the T-cell proteome have implications for anti-cancer therapy.