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PXD000533

DataSet Summary

  • HostingRepository: PRIDE
  • AnnounceDate: 2015-08-12
  • AnnouncementXML: Submission_2015-08-12_02:47:59.xml
  • DigitalObjectIdentifier: http://dx.doi.org/10.6019/PXD000533
  • ReviewLevel: Peer-reviewed dataset
  • DatasetOrigin: Original data
  • RepositorySupport: Supported dataset by repository
  • PrimarySubmitter: Zhang Chengpu Zhang Chengpu
  • Title: CHPP chr 1,8,20 proteome dataset using the HCC cell lines of Hep3B and MHCC97H, instrument is Q Exactive, part 2 of 3
  • Description: The fresh cells as described above were harvested and lysed in buffer containing 8 M Urea, 50 mM NH4HCO3 and 5 mM IAA. The total cell lysates were centrifuged with 12,000 g for 10 min at 4 degrees Celsius to remove cell debris, and 1.5 mg proteins for each cell line were followed by sequential in-solution protein digestion by Lys-C and trypsin at 37 degrees Celsius, respectively. The tryptic peptides were cleaned by C18 Sep-Pak column (Waters UK Ltd, Manchester, UK). The mass spectrometer used in this dataset was Q-Exactive. The dataset was produced with two technical replicates and 24 fractions per repeat. The database searching procedure was achieved using Mascot v2.3. The database is Swiss-prot (release 2013 -6).
  • SpeciesList: scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
  • ModificationList: monohydroxylated residue; iodoacetamide derivatized residue
  • Instrument: instrument model: Q-Exactive

Dataset History

VersionDatetimeStatusChangeLog Entry
02013-10-21 07:03:32ID requested
12013-12-19 02:27:54announced
22015-08-12 02:48:01announcedUpdated publication reference for PubMed record(s): 24328083.

Publication List

  1. Liu Y, Ying W, Ren Z, Gu W, Zhang Y, Yan G, Yang P, Liu Y, Yin X, Chang C, Jiang J, Fan F, Zhang C, Xu P, Wang Q, Wen B, Lin L, Wang T, Du C, Zhong J, Wang T, He QY, Qian X, Lou X, Zhang G, Zhong F, Chromosome-8-coded proteome of Chinese Chromosome Proteome Data set (CCPD) 2.0 with partial immunohistochemical verifications. J Proteome Res, 13(1):126-36(2014) [pubmed]

Keyword List

  1. ProteomeXchange project tag: Chromosome-centric Human Proteome Project (C-HPP), Human Proteome Project
  2. submitter keyword: CHPP, chromesome 1,8,20, HCC cell lines, Q-Exactive

Contact List

    Zhang Chengpu Zhang Chengpu
    • contact affiliation: Beijing proteome research center
    • contact email: zhangchengpu0203@163.com
    • dataset submitter:

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