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PXD000533

PXD000533 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleCHPP chr 1,8,20 proteome dataset using the HCC cell lines of Hep3B and MHCC97H, instrument is Q Exactive, part 2 of 3
DescriptionThe fresh cells as described above were harvested and lysed in buffer containing 8 M Urea, 50 mM NH4HCO3 and 5 mM IAA. The total cell lysates were centrifuged with 12,000 g for 10 min at 4 degrees Celsius to remove cell debris, and 1.5 mg proteins for each cell line were followed by sequential in-solution protein digestion by Lys-C and trypsin at 37 degrees Celsius, respectively. The tryptic peptides were cleaned by C18 Sep-Pak column (Waters UK Ltd, Manchester, UK). The mass spectrometer used in this dataset was Q-Exactive. The dataset was produced with two technical replicates and 24 fractions per repeat. The database searching procedure was achieved using Mascot v2.3. The database is Swiss-prot (release 2013 -6).
HostingRepositoryPRIDE
AnnounceDate2015-08-12
AnnouncementXMLSubmission_2015-08-12_02:47:59.xml
DigitalObjectIdentifierhttp://dx.doi.org/10.6019/PXD000533
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterZhang Chengpu Zhang Chengpu
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
Instrumentinstrument model: Q-Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02013-10-21 07:03:32ID requested
12013-12-19 02:27:54announced
22015-08-12 02:48:01announcedUpdated publication reference for PubMed record(s): 24328083.
Publication List
Liu Y, Ying W, Ren Z, Gu W, Zhang Y, Yan G, Yang P, Liu Y, Yin X, Chang C, Jiang J, Fan F, Zhang C, Xu P, Wang Q, Wen B, Lin L, Wang T, Du C, Zhong J, Wang T, He QY, Qian X, Lou X, Zhang G, Zhong F, Chromosome-8-coded proteome of Chinese Chromosome Proteome Data set (CCPD) 2.0 with partial immunohistochemical verifications. J Proteome Res, 13(1):126-36(2014) [pubmed]
Keyword List
ProteomeXchange project tag: Chromosome-centric Human Proteome Project (C-HPP), Human Proteome Project
submitter keyword: CHPP, chromesome 1,8,20, HCC cell lines, Q-Exactive
Contact List
Zhang Chengpu Zhang Chengpu
contact affiliationBeijing proteome research center
contact emailzhangchengpu0203@163.com
dataset submitter
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