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PXD000527

PXD000527 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleAnalysis of pummelo proteome using iTRAQ
DescriptionFor protein identification, the following parameters were used. Peptide mass tolerance = 20 ppm, Missed cleavage = 2, MS/MS tolerance = 0.1 Da, Enzyme = Trypsin, Fixed modification: Carbamidomethyl (C), iTRAQ8plex(K), iTRAQ8plex(N-term), Variable modification:Oxidation(M),Decoy database pattern=Reverse. The MASCOT search results for each SCX elution were further processed using the ProteomicsTools (version 3.1.6) which includes the programs BuildSummary, Isobaric Labeling Multiple File Distiller and Identified Protein iTRAQ Statistic Builder (Information can be accessed from Research Center for Proteome Analysis (http://www.proteomics.ac.cn/).
HostingRepositoryPRIDE
AnnounceDate2024-10-07
AnnouncementXMLSubmission_2024-10-07_09:10:46.137.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD000527
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterBeibei Zheng
SpeciesList scientific name: Citrus maxima (Pomelo) (Citrus grandis); NCBI TaxID: 37334;
ModificationListiTRAQ8plex-116 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentLTQ Orbitrap
Dataset History
RevisionDatetimeStatusChangeLog Entry
02013-10-17 06:07:03ID requested
12020-01-27 00:33:32announced
22024-10-07 09:10:51announced2024-10-07: Updated project metadata.
Publication List
10.6019/PXD000527;
Zheng BB, Fang YN, Pan ZY, Sun L, Deng XX, Grosser JW, Guo WW, iTRAQ-based quantitative proteomics analysis revealed alterations of carbohydrate metabolism pathways and mitochondrial proteins in a male sterile cybrid pummelo. J Proteome Res, 13(6):2998-3015(2014) [pubmed]
10.1021/pr500126g;
Keyword List
curator keyword: Biological
submitter keyword: iTRAQ floral bud
Contact List
Wen-Wu Guo
contact affiliationKey Laboratory of Horticultural Plant Biology (Ministry of Education), Huazhong Agricultural University , Wuhan 430070, China
contact emailguoww@mail.hzau.edu.cn
lab head
Beibei Zheng
contact affiliationKey Laboratory of Horticultural Plant Biology
contact emailzhengbeibeilove@gmail.com
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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