PXD000527

PXD000527 is an original dataset announced via ProteomeXchange.

Title	Analysis of pummelo proteome using iTRAQ
Description	For protein identification, the following parameters were used. Peptide mass tolerance = 20 ppm, Missed cleavage = 2, MS/MS tolerance = 0.1 Da, Enzyme = Trypsin, Fixed modification: Carbamidomethyl (C), iTRAQ8plex(K), iTRAQ8plex(N-term), Variable modification：Oxidation(M)，Decoy database pattern=Reverse. The MASCOT search results for each SCX elution were further processed using the ProteomicsTools (version 3.1.6) which includes the programs BuildSummary, Isobaric Labeling Multiple File Distiller and Identified Protein iTRAQ Statistic Builder (Information can be accessed from Research Center for Proteome Analysis (http://www.proteomics.ac.cn/).
HostingRepository	PRIDE
AnnounceDate	2024-10-07
AnnouncementXML	Submission_2024-10-07_09:10:46.137.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD000527
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Beibei Zheng
SpeciesList	scientific name: Citrus maxima (Pomelo) (Citrus grandis); NCBI TaxID: 37334;
ModificationList	iTRAQ8plex-116 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2013-10-17 06:07:03	ID requested
1	2020-01-27 00:33:32	announced
⏵ 2	2024-10-07 09:10:51	announced	2024-10-07: Updated project metadata.

10.6019/PXD000527;

Zheng BB, Fang YN, Pan ZY, Sun L, Deng XX, Grosser JW, Guo WW, iTRAQ-based quantitative proteomics analysis revealed alterations of carbohydrate metabolism pathways and mitochondrial proteins in a male sterile cybrid pummelo. J Proteome Res, 13(6):2998-3015(2014) [pubmed]

10.1021/pr500126g;

curator keyword: Biological

submitter keyword: iTRAQ floral bud

Wen-Wu Guo
contact affiliation	Key Laboratory of Horticultural Plant Biology (Ministry of Education), Huazhong Agricultural University , Wuhan 430070, China
contact email	guoww@mail.hzau.edu.cn
lab head
Beibei Zheng
contact affiliation	Key Laboratory of Horticultural Plant Biology
contact email	zhengbeibeilove@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/01/PXD000527

PRIDE project URI

• PRIDE
  1. PXD000527
     1. Label: PRIDE project
     2. Name: Analysis of pummelo proteome using iTRAQ