PXD000527 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Analysis of pummelo proteome using iTRAQ |
Description | For protein identification, the following parameters were used. Peptide mass tolerance = 20 ppm, Missed cleavage = 2, MS/MS tolerance = 0.1 Da, Enzyme = Trypsin, Fixed modification: Carbamidomethyl (C), iTRAQ8plex(K), iTRAQ8plex(N-term), Variable modification:Oxidation(M),Decoy database pattern=Reverse. The MASCOT search results for each SCX elution were further processed using the ProteomicsTools (version 3.1.6) which includes the programs BuildSummary, Isobaric Labeling Multiple File Distiller and Identified Protein iTRAQ Statistic Builder (Information can be accessed from Research Center for Proteome Analysis (http://www.proteomics.ac.cn/). |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-07 |
AnnouncementXML | Submission_2024-10-07_09:10:46.137.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD000527 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Beibei Zheng |
SpeciesList | scientific name: Citrus maxima (Pomelo) (Citrus grandis); NCBI TaxID: 37334; |
ModificationList | iTRAQ8plex-116 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | LTQ Orbitrap |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2013-10-17 06:07:03 | ID requested | |
1 | 2020-01-27 00:33:32 | announced | |
⏵ 2 | 2024-10-07 09:10:51 | announced | 2024-10-07: Updated project metadata. |
Publication List
10.6019/PXD000527; |
Zheng BB, Fang YN, Pan ZY, Sun L, Deng XX, Grosser JW, Guo WW, iTRAQ-based quantitative proteomics analysis revealed alterations of carbohydrate metabolism pathways and mitochondrial proteins in a male sterile cybrid pummelo. J Proteome Res, 13(6):2998-3015(2014) [pubmed] |
10.1021/pr500126g; |
Keyword List
curator keyword: Biological |
submitter keyword: iTRAQ floral bud |
Contact List
Wen-Wu Guo |
contact affiliation | Key Laboratory of Horticultural Plant Biology (Ministry of Education), Huazhong Agricultural University , Wuhan 430070, China |
contact email | guoww@mail.hzau.edu.cn |
lab head | |
Beibei Zheng |
contact affiliation | Key Laboratory of Horticultural Plant Biology |
contact email | zhengbeibeilove@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD000527
- Label: PRIDE project
- Name: Analysis of pummelo proteome using iTRAQ