PXD000525

PXD000525 is an original dataset announced via ProteomeXchange.

Title	Candida albicans SC5314 and RMLU2 secretome and vesicle proteins
Description	The commensal fungus Candida albicans secretes a considerable number of proteins and, as in different fungal pathogens, extracellular vesicles (EVs) have also been observed in the extracellular medium. Our report contains the first proteomic analysis of EVs in C. albicans and a comparative proteomic study with the soluble secreted proteins. To this end, cell-free culture supernatants from C. albicans were harvested by centrifugation, separated into EVs and vesicle-free secretome and analyzed by LC-MS/MS. A total of 96 proteins were identified including 75 and 61 proteins in EVs and vesicle-free secretome, respectively. Out of these, 40 proteins were found in secretome by proteomic analysis for the first time. The soluble proteins were enriched in cell wall and secreted pathogenesis related proteins. Interestingly, more than 90% of these vesicle-free proteins were classical secretory proteins with N-terminal signal peptide, whereas all the leaderless proteins involved in metabolism or the exocytosis and endocytosis process were exclusively cargo of the EVs. We propose a model of the different mechanisms used for C. albicans secreted proteins to reach the extracellular medium. Furthermore, we tested the potential of the Bgl2 protein, identified in vesicles and vesicle-free secretome, to protect against a systemic candidiasis in a murine model.
HostingRepository	PRIDE
AnnounceDate	2015-08-21
AnnouncementXML	Submission_2015-08-21_02:50:14.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Vital Vialas
SpeciesList	scientific name: Candida albicans (Yeast); NCBI TaxID: 5476;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2013-10-17 03:08:17	ID requested
⏵ 1	2015-08-21 02:50:15	announced

Gil-Bona A, Llama-Palacios A, Parra CM, Vivanco F, Nombela C, Monteoliva L, Gil C, Proteomics unravels extracellular vesicles as carriers of classical cytoplasmic proteins in Candida albicans. J Proteome Res, 14(1):142-53(2015) [pubmed]

curator keyword: Biological

submitter keyword: candida albicans secretome vesicles

Vital Vialas
contact affiliation	Unidad de Proteómica UCM-PCM
contact email	vital@farm.ucm.es
lab head
Vital Vialas
contact affiliation	Unidad de Proteómica UCM-PCM
contact email	vital@farm.ucm.es
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/08/PXD000525

PRIDE project URI

• PRIDE
  1. PXD000525
     1. Label: PRIDE project
     2. Name: Candida albicans SC5314 and RMLU2 secretome and vesicle proteins