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PXD000521

PXD000521 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProtein abundance changes and ubiquitylation targets identified upon inhibition of the proteasome with Syringolin A
DescriptionThe numerous roles of the ubiquitin proteasome system (UPS) in cellular control have triggered interest in the identification of these ubiquitylation targets and their sites of ubiquitylation. For the identification of ubiquitylated proteins we employed affinity enrichment using an ubiquitin binding domains (UBAs). A change in the experimental set-up allowed for the identification of proteins that so far have been refractory to identification. We also investigated the changes in protein abundance upon interfering with the UPS by inhibition of the proteasome with the specific inhibitor Syringolin A and using a mutant overexpressing ubiquitin that cannot form K48-linked polyubiquitin chains.
HostingRepositoryPRIDE
AnnounceDate2014-07-24
AnnouncementXMLSubmission_2014-07-24_05:15:54.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD000521
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterKatja Baerenfaller
SpeciesList scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationListiodoacetamide derivatized residue; oxidation
InstrumentLTQ Orbitrap XL
Dataset History
RevisionDatetimeStatusChangeLog Entry
02013-10-16 02:53:59ID requested
12014-04-15 02:37:15announced
22014-05-19 06:48:27announcedUpdated publication reference for PubMed record(s): 24732913.
32014-07-24 05:15:55announcedUpdated project metadata.
Publication List
Svozil J, Hirsch-Hoffmann M, Dudler R, Gruissem W, Baerenfaller K, Protein abundance changes and ubiquitylation targets identified after inhibition of the proteasome with syringolin A. Mol Cell Proteomics, 13(6):1523-36(2014) [pubmed]
Keyword List
curator keyword: Biological
submitter keyword: ubiquitin proteasome system, Syringolin A, ubiquitylation, affinity enrichment, quantitative proteomics
Contact List
Katja Baerenfaller
contact affiliationETH Zurich
contact emailbkatja@ethz.ch
lab head
Katja Baerenfaller
contact affiliationInstitute of Plant Science
contact emailbkatja@ethz.ch
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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