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PXD000520

PXD000520 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleLysine Methylation Promotes VEGFR-2 Activation and Angiogenesis
DescriptionWe have used a chimeric VEGFR-2 in which the extracellular domain of mouse VEGFR-2 was replaced with the extracellular domain of human CSF-1 receptor. VEGFR-2 was immunoprecipitated with anti-VEGFR-2 antibody from PAE cells ectopically expressing VEGFR-2. The immunoprecipitated proteins were eluted and separated on SDS-PAGE, followed by in-gel chymotrypsin or trypsin digestion. The digested samples were analyzed by nano LC/MS/MS on a Thermo Fisher LTQ Orbitrap XL. The LC-MS/MS data were analyzed using Proteome Discoverer (Thermo Fisher Scientific; Version 1.3.0.339). MS/MS search was carried out using Sequest search algorithm against the sequence of target mouse protein from the UniProtKB database. Search parameters included chymotrypsin as the enzyme with four missed cleavage allowed; methylation at lysine and arginine, phosphorylation of serine, threonine, and tyrosine, alkylation at cysteine, and oxidation of methionine were set as dynamic modifications. Precursor and fragment mass tolerance were set to 5 ppm and 0.8 Da, respectively. The false discovery rate was calculated by enabling the peptide sequence analysis using a decoy database. High confidence peptide identifications were obtained by setting a target false discovery rate threshold of 1% at the peptide level.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:59:31.826.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterYan Jiang
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonomethylated residue; phosphorylated residue; N6,N6,N6-trimethyl-L-lysine; monohydroxylated residue; dimethylated residue; iodoacetamide derivatized residue
InstrumentLTQ Orbitrap
Dataset History
RevisionDatetimeStatusChangeLog Entry
02013-10-16 01:04:48ID requested
12021-03-04 11:10:25announced
22024-10-22 04:59:36announced2024-10-22: Updated project metadata.
Publication List
10.1126/scisignal.2004289;
Hartsough EJ, Meyer RD, Chitalia V, Jiang Y, Marquez VE, Zhdanova IV, Weinberg J, Costello CE, Rahimi N, Lysine methylation promotes VEGFR-2 activation and angiogenesis. Sci Signal, 6(304):ra104(2013) [pubmed]
Keyword List
curator keyword: Biological
submitter keyword: in-gel digestion, LC-MS/MS
Contact List
Nader Rahimi
contact affiliationLysine Methylation Promotes VEGFR-2 Activation and Angiogenesis
contact emailNader.Rahimi@bmc.org
lab head
Yan Jiang
contact affiliationDepartment of Biochemistry
contact emailyanjiang@bu.edu
dataset submitter
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Dataset FTP location
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PRIDE project URI
Repository Record List
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