PXD000514

PXD000514 is an original dataset announced via ProteomeXchange.

Title	Proteomic Analysis of olfactory cilia membranes
Description	Membranes of olfactory cilia were isolated from mouse olfactory epithelia. Sample fractionation was carried out in parallel by two methods: Proteins were fractionated by SDS-PAGE, the gel lanes were cut into bands and the proteins digested in-gel. Alternatively, proteins were digested in solution and peptides fractionated by strong cation exchange chromatography. Samples were analyzed using an LTQ-Orbitrap Velos. Mass spectrometric data of all replicates were searched together using the software MaxQuant (version 1.2.0.18) and its integrated search engine Andromeda against the UniProt reference proteome set for Mus musculus (http://www.uniprot.org/taxonomy/complete-proteomes; version of March 2, 2012, containing 54,231 entries) appended with a list of common contaminant proteins provided by MaxQuant. Database searches were performed with tryptic specificity allowing two missed cleavages and 7 ppm initial mass tolerance for precursor ions and 0.5 Da for fragment ions. Oxidation of methionine was considered as variable modification; fixed modifications were not included. Peptides and proteins were identified with a false discovery rate of 0.01.
HostingRepository	PRIDE
AnnounceDate	2017-01-17
AnnouncementXML	Submission_2017-01-17_13:23:57.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Gerhard Mayer
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	L-methionine sulfoxide
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2013-10-14 01:48:24	ID requested
⏵ 1	2017-01-17 13:24:08	announced

Kuhlmann K, Tschapek A, Wiese H, Eisenacher M, Meyer HE, Hatt HH, Oeljeklaus S, Warscheid B, The membrane proteome of sensory cilia to the depth of olfactory receptors. Mol Cell Proteomics, 13(7):1828-43(2014) [pubmed]

submitter keyword: Cilium, membrane, olfactory sensory neurons, LC-M

Prof. Dr. Katrin Marcus
contact affiliation	Medizinisches Proteom-Center, Ruhr-Universität Bochum, ZKF 1.055, Universitätsstraße 150, 44801 Bochum, Germany
contact email	Katrin.Marcus@rub.de
lab head
Gerhard Mayer
contact affiliation	Bioinformatics / Biostatistics
contact email	mayerg97@ruhr-uni-bochum.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/01/PXD000514

PRIDE project URI

• PRIDE
  1. PXD000514
     1. Label: PRIDE project
     2. Name: Proteomic Analysis of olfactory cilia membranes