PXD000512

PXD000512 is an original dataset announced via ProteomeXchange.

Title	Mouse aging oocyte proteome comparison
Description	Here we asked if oocyte proteomes are representative of the transcriptomes, how the abundance of specific genes’ mRNA and protein responds to maternal aging, and if oocyte aging presents the features characteristic of somatic aging. To address these questions on the proteomic level, we employed stable isotope labeling of amino acids in cell culture (SILAC; Geiger et al. 2011) as the method of choice, and performed a SILAC screen of mouse metaphase II (MII) oocytes superovulated at 3, 8+-1 and 58+-10 weeks of maternal age, which correspond to pre-puberty, mature age and climacterium, respectively. We used heavy F9 embryonic carcinoma (EC) cells as internal or "Spike-in" standard for the quantification of oocytes proteins because in contrast to the oocytes they can easily be cultured feeder-free, have stem cell properties and should harbor the majority of all oocyte proteins (although with different relative abundances). The SILAC screen was conducted in parallel with conventional microarray analysis to compare the concordance of protein and transcript levels in these oocytes. Associated microarray data have been deposited to NCBI GEO with accession number GSE42959.
HostingRepository	PRIDE
AnnounceDate	2016-10-14
AnnouncementXML	Submission_2016-10-14_04:37:51.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Hannes Drexler
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2013-10-11 05:11:25	ID requested
1	2016-10-14 03:41:35	announced
⏵ 2	2016-10-14 04:37:52	announced	Updated project metadata.

Schwarzer C, Siatkowski M, Pfeiffer MJ, Baeumer N, Drexler HC, Wang B, Fuellen G, Boiani M, Maternal age effect on mouse oocytes: new biological insight from proteomic analysis. Reproduction, 148(1):55-72(2014) [pubmed]

curator keyword: Biological

submitter keyword: Mus musculus, oocyte, embryonal carcinoma cells, SILAC, Spike-in

Hannes C.A. Drexler
contact affiliation	Max Planck Institute for Molecular Biomedicine, Bioanalytical Mass Spectrometry, Röntgenstr. 20, D-48149 Münster Germany
contact email	hannes.drexler@mpi-muenster.mpg.de
lab head
Hannes Drexler
contact affiliation	Bioanalytical Mass Spectrometry
contact email	hannes.drexler@mpi-muenster.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/10/PXD000512

PRIDE project URI

• PRIDE
  1. PXD000512
     1. Label: PRIDE project
     2. Name: Mouse aging oocyte proteome comparison