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PXD000511

PXD000511 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleCdh1 Proteomic Analysis
DescriptionThe Anaphase-promoting complex/cyclosome (APC/C) cofactor Cdh1 modulates proliferation and cell cycle exit by targeting multiple cell cycle regulators for ubiquitin-dependent degradation. Lack of Cdh1 results in structural and numerical chromosome aberrations, a hallmark of genomic instability, although the specific targets involved in these defects are not well understood. Here, we use MS-based proteomics to identify substrates of Cdh1 important for cell cycle regulation. To this end, we performed three experiments: 1 - SILAC analysis of Cdh1 deficient asynchronous MEFs (samples were fractionated by means of SDS-PAGE and IEF) 2 - iTRAQ analysis of Cdh1 deficient synchronous MEFs (samples were fractionated by means of IEF) 3 - iTRAQ analysis of brain tissue in a Cdh1 deficient Mouse (samples were fractionated by means of IEF) LC-MS was carried out using a LTQ-Orbitrap Velos as described in Eguren et al. iTRAQ raw data was processed using Proteome Discoverer 1.3 interfaced with Mascot 2.2 SILAC raw data was processed using MaxQuant suite using Andromeda as search engine Further details can be found in Eguren et al.
HostingRepositoryPRIDE
AnnounceDate2014-07-25
AnnouncementXMLSubmission_2014-07-25_01:42:11.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterFernando Garcia
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList6x(13)C: 4x(15)N labeled L-arginine; monohydroxylated residue; iTRAQ4plex-116 reporter+balance reagent acylated residue; iodoacetamide derivatized residue; 6x(13)C: 2x(15)N labeled L-lysine
InstrumentLTQ Orbitrap Velos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02013-10-11 02:11:22ID requested
12014-02-20 06:02:17announced
22014-07-25 01:42:12announcedUpdated project metadata.
Publication List
Eguren M, Á, lvarez-Fern, á, ndez M, Garc, í, a F, L, ó, pez-Contreras AJ, Fujimitsu K, Yaguchi H, Luque-Garc, í, a JL, Fern, á, ndez-Capetillo O, Mu, ñ, oz J, Yamano H, Malumbres M, A synthetic lethal interaction between APC/C and topoisomerase poisons uncovered by proteomic screens. Cell Rep, 6(4):670-83(2014) [pubmed]
Keyword List
submitter keyword: Cdh1, APC/C, Cell cycle, Ubiquitination, SILAC, iTRAQ, mouse
Contact List
Fernando Garcia
contact affiliationProteomics Group - Spanish National Cancer Research Center (CNIO)
contact emailfgarcia@cnio.es
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
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