PXD000507

PXD000507 is an original dataset announced via ProteomeXchange.

Title	Acetylation Dynamics and Stoichiometry in Saccharomyces cerevisiae
Description	Quantitative MS analysis of acetylation in yeast using SILAC labeling and MaxQuant. Download Index of Raw files first. We used quantitative mass spectrometry to analyze acetylation dynamics and stoichiometry in Saccharomyces cerevisiae. We found that acetylation accumulated in growth-arrested cells in a manner that depended on acetyl-CoA generation in distinct subcellular compartments. We used stable isotope labeling with amino acids in cell culture to quantify differences in protein, acetylation, and phosphorylation abundance by MS. Proteins from whole cell lysates were digested to peptides and acetylated peptides enriched using a polyclonal anti-acetyllysine antibody. Peptide fractions were analyzed by reversed-phase liquid chromatography coupled to high resolution liquid chromatography‐tandem mass spectrometry (LC-MS/MS) and raw MS data were computationally processed using MaxQuant.
HostingRepository	PRIDE
AnnounceDate	2014-07-24
AnnouncementXML	Submission_2014-07-24_03:50:18.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Brian Weinert
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	acetylated residue; phosphorylated residue
Instrument	LTQ Orbitrap Velos; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2013-10-09 05:50:46	ID requested
1	2014-02-03 07:56:19	announced
2	2014-03-05 07:29:35	announced	Updated publication reference for PubMed record(s): 24489116.
⏵ 3	2014-07-24 03:50:19	announced	Updated project metadata.

Weinert BT, Iesmantavicius V, Moustafa T, Sch, ö, lz C, Wagner SA, Magnes C, Zechner R, Choudhary C, Acetylation dynamics and stoichiometry in Saccharomyces cerevisiae. Mol Syst Biol, 10(1):716(2014) [pubmed]

ProteomeXchange project tag: PRIME-XS Project

submitter keyword: acetylation, stoichiometry, mitochondria, metabolism, yeast

Brian Weinert
contact affiliation	Proteomics
contact email	brtw@cpr.ku.dk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2014/02/PXD000507

PRIDE project URI

• PRIDE
  1. PXD000507
     1. Label: PRIDE project
     2. Name: Acetylation Dynamics and Stoichiometry in Saccharomyces cerevisiae