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PXD000500-2

PXD000500 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleArabidopsis mitochondrial proteome during a diurnal cycle
DescriptionWe surveyed the heterogeneity of the mitochondrial proteome and its function during a typical night and day cycle in Arabidopsis shoots. This used a staged, quantitative analysis of the proteome across 10 time points covering 24 h of the life of 3-week-old Arabidopsis shoots grown under 12-h dark and 12-h light conditions. Results were queried against an in-house Arabidopsis database comprising ATH1.pep (release 7) from The Arabidopsis Information Resource and the Arabidopsis mitochondrial and plastid protein sets (the combined database contained a total of 30,700 protein sequences with 12,656,682 residues) using the Mascot search engine version 2.2 and utilizing error tolerances of +-1.2 Da for MS and +-0.6 Da for MS/MS, 'enzyme' set to trypsin, 'maximum missed cleavages' set to 1, variable modifications of oxidation (Met) and carbamidomethyl (Cys), instrument set to ESI-TRAP, and peptide charge set at 2+ and 3+. ATH1.pep is a non-redundant database with systematically named protein sequences based on Arabidopsis genome sequencing and annotation. PRIDE XML files with accession numbers 10471–10525 and without peptide/protein identifications have already been made public.
HostingRepositoryPRIDE
AnnounceDate2014-07-28
AnnouncementXMLSubmission_2014-07-28_00:49:45.xml
DigitalObjectIdentifierhttp://dx.doi.org/10.6019/PXD000500
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterChun Pong Lee
SpeciesList scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationListmonohydroxylated residue; iodoacetic acid derivatized residue; iodoacetamide derivatized residue
Instrumentinstrument model: IonTrap XCT Ultra
Dataset History
RevisionDatetimeStatusChangeLog Entry
02013-10-07 07:49:32ID requested
12013-10-09 02:10:48announced
12013-10-09 02:41:19announced
22014-07-28 00:49:46announcedUpdated project metadata.
Publication List
Lee CP, Eubel H, Millar AH, Diurnal changes in mitochondrial function reveal daily optimization of light and dark respiratory metabolism in Arabidopsis. Mol Cell Proteomics, 9(10):2125-39(2010) [pubmed]
Keyword List
submitter keyword: Arabidopsis, plant, mitochondria, diurnal cycle, LC-MS/MS
Contact List
Chun Pong Lee
contact affiliationDepartment of Plant Sciences, University of Oxford
contact emailchun.lee@plants.ox.ac.uk
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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