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PXD000500-1

PXD000500 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleArabidopsis mitochondrial proteome during a diurnal cycle
DescriptionWe surveyed the heterogeneity of the mitochondrial proteome and its function during a typical night and day cycle in Arabidopsis shoots. This used a staged, quantitative analysis of the proteome across 10 time points covering 24 h of the life of 3-week-old Arabidopsis shoots grown under 12-h dark and 12-h light conditions. Results were queried against an in-house Arabidopsis database comprising ATH1.pep (release 7) from The Arabidopsis Information Resource and the Arabidopsis mitochondrial and plastid protein sets (the combined database contained a total of 30,700 protein sequences with 12,656,682 residues) using the Mascot search engine version 2.2 and utilizing error tolerances of +-1.2 Da for MS and +-0.6 Da for MS/MS, 'enzyme' set to trypsin, 'maximum missed cleavages' set to 1, variable modifications of oxidation (Met) and carbamidomethyl (Cys), instrument set to ESI-TRAP, and peptide charge set at 2+ and 3+. ATH1.pep is a non-redundant database with systematically named protein sequences based on Arabidopsis genome sequencing and annotation. PRIDE XML files with accession numbers 10471–10525 and without peptide/protein identifications have already been made public.
HostingRepositoryPRIDE
AnnounceDate2013-10-09
AnnouncementXMLSubmission_2013-10-09_02:10:39.xml
DigitalObjectIdentifierhttp://dx.doi.org/10.6019/PXD000500
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterChun Pong Lee
SpeciesList scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationListmonohydroxylated residue; neutral loss: 63.998285; iodoacetic acid derivatized residue; iodoacetamide derivatized residue
Instrumentselected ion m/z
Dataset History
RevisionDatetimeStatusChangeLog Entry
02013-10-07 07:49:32ID requested
12013-10-09 02:10:48announced
12013-10-09 02:41:19announced
22014-07-28 00:49:46announcedUpdated project metadata.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Arabidopsis, plant, mitochondria, diurnal cycle, LC-MS/MS
Contact List
Chun Pong Lee
contact affiliationDepartment of Plant Sciences, University of Oxford
contact emailchun.lee@plants.ox.ac.uk
Prof. A. Harvey Millar
contact attributeharvey.millar@uwa.edu.au
contact affiliationARC CoE Plant Energy Biology, University of Western Australia
contact emailharvey.millar@uwa.edu.au
Dr. Chun Pong Lee
contact attributecplee@graduate.uwa.edu.au
contact affiliationARC CoE Plant Energy Biology, University of Western Australia
contact emailcplee@graduate.uwa.edu.au
Full Dataset Link List
Dataset FTP location
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Repository Record List
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