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PXD000499

PXD000499 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitlemiRNA-mediated regulation of hBC cell proteome
DescriptionEstrogen Receptor B (ERB) is a member of the nuclear receptor family of homeostatic regulators that is frequently lost in breast cancer (BC), where its presence correlates with a better prognosis and a less aggressive clinical outcome of the disease. Contrary to ERα, its closest homolog, ERB shows also significant estrogen-independent activities, including the ability to inhibit cell cycle progression and to regulate gene transcription in the absence of the ligand. Investigating the nature and extent of this constitutive activity of ERB in BC MCF-7 cells by miRNA-Seq, we identified 127 miRNAs differentially expressed in ERB+ vs ERB- cells in the absence of ligand, including upregulated oncosuppressor miRs, such miR-30a, and downregulated onco-miRs, like miR-21. In addition, a significant fraction of >1,600 unique proteins identified in these cells by iTRAQ (isobaric Tag for Relative and Absolute Quantitation) quantitative proteomics was either increased or decreased by ERB, revealing regulation of multiple cell pathways by ligand-free receptor. Transcriptome analysis indicates that for a large number of proteins regulated by ERB the corresponding mRNAs are unaffected, including a large number of putative targets of ERB-regulated miRNAs, indicating a central role of miRNAs in mediating BC cell proteome regulation by ERB. Expression of a mimic of miR-30a-5p, a direct target and downstream effector of ERB in BC, led to the identification of several target transcripts of this miRNA, including 11 encoding proteins whose intracellular concentration is significantly affected by unliganded receptor. These results demonstrate a significant effect of ligand-free ERB on BC cell functions via modulation of the cell proteome and suggest that miRNA regulation may represent a key event in the control of the biological and clinical phenotype of hormone-responsive BC by this nuclear receptor.
HostingRepositoryPRIDE
AnnounceDate2014-10-23
AnnouncementXMLSubmission_2014-10-23_02:39:00.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterTuula Nyman
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListiTRAQ4plex-116 reporter+balance reagent acylated residue; methylthiolated residue
InstrumentQSTAR
Dataset History
RevisionDatetimeStatusChangeLog Entry
02013-10-07 07:42:14ID requested
12014-10-23 01:24:38announced
22014-10-23 02:39:01announcedUpdated project metadata.
Publication List
Nassa G, Tarallo R, Giurato G, De Filippo MR, Ravo M, Rizzo F, Stellato C, Ambrosino C, Baumann M, Lietz, è, n N, Nyman TA, Weisz A, via microRNAs. Mol Cell Proteomics, 13(4):1076-90(2014) [pubmed]
Keyword List
curator keyword: Biomedical
submitter keyword: miRNA, estrogen receptor beta, breast cancer cell proteome, iTRAQ
Contact List
Tuula Nyman
contact affiliationInstitute of Biotechnology
contact emailtuula.nyman@helsinki.fi
dataset submitter
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Dataset FTP location
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