PXD000492

PXD000492 is an original dataset announced via ProteomeXchange.

Title	CDK regulated chicken chromatin
Description	Initiation and completion of DNA replication has a major impact on chromatin, but how core chromatin processes are cell cycle regulated in somatic cells remains poorly understood. To address this question we conducted a quantitative proteomics study that took advantage of an analogue sensitive mutation in Cdk1 (cdk1as), which allows for rapid and highly specific inactivation of Cdk1 by the bulky ATP analogue 1NMPP1. Neither Cdk1 nor Cdk2 are required for S-phase progression, while inactivation of Cdk1 in Cdk2 knock-out cells causes a complete block of DNA replication initiation and S-phase progression. Here we compared chromatin fractions obtained from cdk1as and cdk1as/cdk2-/- cells two hours after Cdk1 inactivation. Statistical analysis of these four independent experiments (including 2 label swaps) identified 135 proteins that showed a significant change in SILAC ratio among a total of 2402 proteins quantified in at least three experiments. This approach led us to discover a novel functional interplay between interphase Cdks and the chromatin association of a variety of novel candidate proteins such as Cdk1 itself, the APC/C regulatory subunit cdc20, the PP4 regulatory subunit Smek2, the helicase FUBP1 and the PHD domain-containing zinc finger protein PHF6.
HostingRepository	PRIDE
AnnounceDate	2014-07-24
AnnouncementXML	Submission_2014-07-24_05:16:51.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Georg Kustatscher
SpeciesList	scientific name: Gallus gallus (Chicken); NCBI TaxID: 9031;
ModificationList	iodoacetamide derivatized residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2013-10-03 07:34:49	ID requested
1	2014-01-29 02:54:25	announced
2	2014-04-10 05:51:49	announced	Updated publication reference for PubMed record(s): 24534090.
⏵ 3	2014-07-24 05:16:52	announced	Updated project metadata.

Kustatscher G, H, é, garat N, Wills KL, Furlan C, Bukowski-Wills JC, Hochegger H, Rappsilber J, Proteomics of a fuzzy organelle: interphase chromatin. EMBO J, 33(6):648-64(2014) [pubmed]

curator keyword: Biological

submitter keyword: Chicken, DT40, CDK, Chromatin

Juri Rappsilber
contact affiliation	Wellcome Trust Centre for Cell Biology
contact email	juri.rappsilber@ed.ac.uk
lab head
Georg Kustatscher
contact affiliation	Wellcome Trust Centre for Cell Biology
contact email	georg.kustatscher@ed.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2014/01/PXD000492

PRIDE project URI

• PRIDE
  1. PXD000492
     1. Label: PRIDE project
     2. Name: CDK regulated chicken chromatin