PXD000489

PXD000489 is an original dataset announced via ProteomeXchange.

Title	A pair of centromeric proteins mediates reproductive isolation in Drosophila species
Description	Speciation involves the reproductive isolation of natural populations due to the sterility or lethality of their hybrids. However, the molecular basis of hybrid lethality and the evolutionary driving forces that provoke it, remain largely elusive. The hybrid male rescue (Hmr) and the lethal hybrid rescue (Lhr) genes serve as a model to study speciation in Drosophilids as their interaction causes lethality in male hybrid offspring. Here we show that HMR and LHR form a centromeric complex necessary for proper chromosome segregation. We find that the Hmr expression level is substantially higher in D. melanogaster whereas Lhr expression levels are increased in D. simulans. The resulting elevated amount of HMR/LHR complex in hybrids results in an extensive mislocalisation of the complex, an interference with the regulation of transposable elements and an impairment of cell proliferation. Our findings provide evidence for a major role of centromere divergence in the generation of biodiversity. Raw data were analysed using the MaxQuant 1.2.2.5 software package. Identified proteins were considered as interation partners if their MaxQuant iBAQ values displayed a greater than 16fold enrichment compared to control anti-FLAG purifications from Schneider cell nuclear extracts not expressing any FLAG-tagged protein.
HostingRepository	PRIDE
AnnounceDate	2014-08-08
AnnouncementXML	Submission_2014-08-08_00:31:19.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Andreas Thomae
SpeciesList	scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationList	iodoacetamide derivatized residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2013-10-02 07:09:54	ID requested
1	2013-11-20 00:37:34	announced
1	2013-11-20 00:38:19	announced
2	2014-07-25 01:40:01	announced	Updated project metadata.
⏵ 3	2014-08-08 00:31:20	announced	Updated project metadata.

Thomae AW, Schade GO, Padeken J, Borath M, Vetter I, Kremmer E, Heun P, Imhof A, A pair of centromeric proteins mediates reproductive isolation in Drosophila species. Dev Cell, 27(4):412-24(2013) [pubmed]

curator keyword: Biological

submitter keyword: HMR, LHR

Andreas Thomae
contact affiliation	Molecular Biology
contact email	andreas.thomae@med.uni-muenchen.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2013/11/PXD000489

PRIDE project URI

• PRIDE
  1. PXD000489
     1. Label: PRIDE project
     2. Name: A pair of centromeric proteins mediates reproductive isolation in Drosophila species